Cat. No.: RF16020

PD-L2 Mouse mAb Detection Set (Risk-Free™)

PD-L2 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PD-L2 can act to stimulate an immunogenic response through an alternative receptor from PD-1.

Each Risk-Free™ monoclonal antibody set consists of several 20μg trial size mAbs with different binding features to help you find the best clone for your specific application. Go to set contents.

ProSci Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications.


Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells.

(Click image to expand)
Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells. See high quality image.

Western blot analysis of PD-L2 in overexpressing 293 cells using RF16021, RF16022, RF16023, RF16024, and RF16025 antibody at 0.5 and 1 μg/ml, respectively. Larger molecular weight bands represent more highly post-translationally modified PD-L2

(Click image to expand)
Western blot analysis of PD-L2 in overexpressing 293 cells using RF16021, RF16022, RF16023, RF16024, and RF16025 antibody at 0.5 and 1 μg/ml, respectively. Larger molecular weight bands represent more highly post-translationally modified PD-L2(4). See high quality image.

Immunofluorescence of PD-L2 in overexpressing 293 cells using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 20 μg/ml

(Click image to expand)
Immunofluorescence of PD-L2 in overexpressing 293 cells using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 20 μg/ml. See high quality image.

Immunofluorescence of PD-L2 in human tonsil tissue using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 20 μg/ml

(Click image to expand)
Immunofluorescence of PD-L2 in human tonsil tissue using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 20 μg/ml. See high quality image.

Immunohistochemistry of PD-L2 in human colon carcinoma tissue using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 2 μg/ml

(Click image to expand)
Immunohistochemistry of PD-L2 in human colon carcinoma tissue using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 2 μg/ml. See high quality image.

Immunohistochemistry of PD-L2 in human tonsil tissue using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 2 μg/ml

(Click image to expand)
Immunohistochemistry of PD-L2 in human tonsil tissue using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 2 μg/ml. See high quality image.

Immunocytochemistry of PD-L2 in overexpressing 293 cells using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 5 μg/ml

(Click image to expand)
Immunocytochemistry of PD-L2 in overexpressing 293 cells using (A) RF16021, (B) RF16022, (C) RF16023, (D) RF16024, (E) RF16025, and (F) control mouse IgG antibody at 5 μg/ml. See high quality image.

Titration curve analysis of PD-L2 mAbs to detect recombinant PD-L2 in ELISA with RF16021, RF16022, RF16023, RF16024, and RF16025 antibodies at decreasing concentrations

(Click image to expand)
Titration curve analysis of PD-L2 mAbs to detect recombinant PD-L2 in ELISA with RF16021, RF16022, RF16023, RF16024, and RF16025 antibodies at decreasing concentrations. See high quality image.

PD-L2 Mouse Monoclonal Set Contents

(Click image to expand)
PD-L2 Mouse Monoclonal Set Contents.

psi-iconSpecifications

NamePD-L2 Detection Set (Risk-Free™)
Alternate Names PD-L2 Antibody: B7DC, Btdc, PDL2, CD273, PD-L2, PDCD1L2, bA574F11.2, B7DC, Programmed cell death 1 ligand 2, Butyrophilin B7-DC, PD-1 ligand 2
Host Species Mouse
Tested Applications ELISA, Flow, ICC, IF, IHC-P, WB
Immunogen PD-L2 antibody was raised against the extracellular domain of human PD-L2.
Size 20 μg per clone

psi-iconProperties

Clonality Monoclonal
Isotype IgG1 and IgG2b
Conjugate Unconjugated
Physical State Liquid
Buffer PD-L2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration 1 mg/mL
Storage Conditions Antibodies can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconSet Contents

Hover over application title for rating comparison image.

= Negative = Fair   = Average    = Good     = Excellent= Best Application
Cat. No.ELISAELISA Titration Curve Analysis
ELISA Titration Curve Analysis
WBWestern Blot in HEK293 Cells
Western Blot in HEK293 Cells
ICC
ICC in HEK293 Cells
IF
IF in HEK293 Cells
IHCIHC in Human Tonsil Tissue
IHC in Human Tonsil Tissue
FlowFlow Cytometry in HEK293 Cells
Flow Cytometry in HEK293 Cells
RF16021                
RF16022                     
RF16023                     
RF16024                    
RF16025                    

psi-iconProduct Specific Information

ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vitro studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. These antibodies have the following advantages:

  • Antibodies are developed against mammalian-expressed, post-translationally modified recombinant proteins.
  • Multiple antibodies per target allows the user to choose the best antibody for their application
  • Available individually or as a set
  • Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications:
    • • ELISA• Flow Cytometry• Immunoblot • Immunohistochemistry
      • Immunocytochemistry • Immunofluorescence

psi-iconBackground

PD-L2 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PD-L2 can act to stimulate an immunogenic response through an alternative receptor from PD-1.


psi-iconReferences

1) Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
2) Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
3) LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.
4) Yamazaki T, Akiba H, Iwai H, et al. Expression of programmed death 1 ligands by murine T cells and APC. J. Immunol. 2002; 169:5538-45. 5) Zhang Y, Chung Y, Bishop C, et al. Regulation of T cell activation and tolerance by PDL2. Proc. Natl. Acad. Sci. USA 2006; 103:11695-700.


ANTIBODIES FOR RESEARCH USE ONLY.

For additiona information, visit ProSci's Terms & Conditions Page.

 Price Options

1 Set - $299.00

P4 Members save 10%!

Set consists of 20μg per clone.

 Shipping

$55 FedEx Standard Overnight flat rate for as many products shipped within the USA.
$29 Dry Ice Shipment fee may be required on some items.
Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

 Contact Us

Toll Free: (888) 513 9525
Local: (858) 513 2638
Fax: (858) 513 2692

orders@prosci-inc.com