Cat. No.:RF16030

PD-L1 Mouse mAb Detection Set (Risk-Free™)

PD-L1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) (1). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC (2). PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells (3). This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. PD-L1 also functions as an immune checkpoint protein, and multiple anti-PD-L1 antibodies are currently in phase II and III clinical trials, with one antibody already approved for the treatment of cancer (4).

Each Risk-Free™ monoclonal antibody set consists of several 20μg trial size mAbs with different binding features to help you find the best clone for your specific application. Go to set contents.

ProSci Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications.


Flow cytometry analysis of PD-L1 overexpressing HEK293 cells using (A) RF16031, (B) RF16032, and (C) control mouse IgG antibody at 10 μg/ml.  Blue:  Untransfected HEK293 cells.  Yellow:  PD-L1 overexpressing HEK293 cells

(Click image to expand)
Flow cytometry analysis of PD-L1 overexpressing HEK293 cells using (A) RF16031, (B) RF16032, and (C) control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L1 overexpressing HEK293 cells. See high quality image.

Western blot analysis of PD-L1 in overexpressing HEK293 cells using RF16031, RF16032, RF16035, RF16036, RF16037, and RF16038 antibody at 0.25 and 0.5 μg/ml

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Western blot analysis of PD-L1 in overexpressing HEK293 cells using RF16031, RF16032, RF16035, RF16036, RF16037, and RF16038 antibody at 0.25 and 0.5 μg/ml, respectively. Larger molecular weight bands represent more highly post-translationally modified PD-L1. See high quality image.

Immunofluorescence of PD-L1 in overexpressing HEK293 cells using (A) polyclonal PD-L1 antibody, (B) RF16031, (C) RF16032, (D) RF16035, (E) RF16036, (F) RF16037, (G) RF16038 and (H) control mouse IgG antibody at 2 μg/ml.

(Click image to expand)
Immunofluorescence of PD-L1 in overexpressing HEK293 cells using (A) polyclonal PD-L1 antibody, (B) RF16031, (C) RF16032, (D) RF16035, (E) RF16036, (F) RF16037, (G) RF16038 and (H) control mouse IgG antibody at 2 μg/ml. See high quality image.

Immunofluorescence of PD-L1 in human stomach carcinoma tissue using (A) polyclonal PD-L1 antibody at 20 μg/ml, (B) RF16031, (C) RF16032, (D) RF16035, (E) RF16036, (F) RF16037, (G) RF16038 and (H) control mouse IgG antibody at 2 μg/ml

(Click image to expand)
Immunofluorescence of PD-L1 in human stomach carcinoma tissue using (A) polyclonal PD-L1 antibody at 20 μg/ml, (B) RF16031, (C) RF16032, (D) RF16035, (E) RF16036, (F) RF16037, (G) RF16038 and (H) control mouse IgG antibody at 2 μg/ml. See high quality image.

Immunofluorescence of PD-L1 in human tonsil tissue using (A) polyclonal PD-L1 antibody, (B) RF16031, (C) RF16032, (D) RF16035, (E) RF16036, (F) RF16037, (G) RF16038 and (H) control mouse IgG antibody at 2 μg/ml

(Click image to expand)
Immunofluorescence of PD-L1 in human tonsil tissue using (A) polyclonal PD-L1 antibody, (B) RF16031, (C) RF16032, (D) RF16035, (E) RF16036, (F) RF16037, (G) RF16038 and (H) control mouse IgG antibody at 2 μg/ml. See high quality image.

Immunohistochemistry of PD-L1 in human stomach carcinoma tissue using (A) polyclonal PD-L1 antibody, (B) RF16031, (C) RF16032, (D) RF16035, (E) RF16036, (F) RF16037, (G) RF16038 and (H) control mouse IgG antibody at 5 μg/ml

(Click image to expand)
Immunohistochemistry of PD-L1 in human stomach carcinoma tissue using (A) polyclonal PD-L1 antibody, (B) RF16031, (C) RF16032, (D) RF16035, (E) RF16036, (F) RF16037, (G) RF16038 and (H) control mouse IgG antibody at 5 μg/ml. See high quality image.

Immunohistochemistry of PD-L1 in human tonsil tissue using (A) polyclonal PD-L1 antibody, (B) RF16031, (C) RF16032, (D) RF16035, (E) RF16036, (F) RF16037, (G) RF16038 and (H) control mouse IgG antibody at 5 μg/ml

(Click image to expand)
Immunohistochemistry of PD-L1 in human tonsil tissue using (A) polyclonal PD-L1 antibody, (B) RF16031, (C) RF16032, (D) RF16035, (E) RF16036, (F) RF16037, (G) RF16038 and (H) control mouse IgG antibody at 5 μg/ml. See high quality image.

(Click image to expand)
Immunocytochemistry of PD-L1 in overexpressing HEK293 cells using (A) polyclonal PD-L1 antibody at 2 μg/ml, (B) RF16031, (C) RF16032, (D) RF16035, (E) RF16036, (F) RF16037, (G) RF16038 and (H) control mouse IgG antibody at 1 μg/ml. See high quality image.

Titration curve analysis of PD-L1 mAbs to detect recombinant PD-L1 in ELISA with RF16031, RF16032, RF16035, RF16036, RF16037, and RF16038 abs at decreasing concentrations

(Click image to expand)
Titration curve analysis of PD-L1 mAbs to detect recombinant PD-L1 in ELISA with RF16031, RF16032, RF16035, RF16036, RF16037, and RF16038 abs at decreasing concentrations. See high quality image.

psi-iconSpecifications

NamePD-L1 Detection Set (Risk-Free™)
Alternate Names PD-L1 Antibody: Programmed cell death 1 ligand-1, programmed death ligand 1, PDL1, PDL-1, B7-H1
Host Species Mouse
Tested Applications ELISA, Flow, ICC, IF, IHC-P, WB
Immunogen Mouse monoclonal PD-L1 antibodies were raised against the extracellular domain of human PD-L1.
Size 20 μg per clone

psi-iconProperties

Clonality Monoclonal
Isotype IgG1
Conjugate Unconjugated
Physical State Liquid
Buffer PBS containing 0.02% sodium azide.
Concentration 1 mg/mL
Storage Conditions Antibodies can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconSet Contents

Hover over application title for rating comparison image.

= Negative = Fair   = Average    = Good     = Excellent= Best Application
Cat. No.ELISAELISA Titration Curve Analysis
ELISA Titration Curve Analysis
WBWestern Blot in HEK293 Cells
Western Blot in HEK293 Cells
ICCICC in HEK293 Cells
ICC in HEK293 Cells
IFIF in HEK293 Cells
IF in HEK293 Cells
IHCIHC
IHC in Human Tonsil Tissue
FlowFlow Cytometry in HEK293 Cells
Flow Cytometry in HEK293 Cells
RF16031                    
RF16032                   
RF16035                   
RF16036                    
RF16037                    
RF16038                      

psi-iconProduct Specific Information

ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vitro studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. These antibodies have the following advantages:

  • Antibodies are developed against mammalian-expressed, post-translationally modified recombinant proteins.
  • Multiple antibodies per target allows the user to choose the best antibody for their application
  • Available individually or as a set
  • Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications:
    • • ELISA• Flow Cytometry• Immunoblot • Immunohistochemistry
      • Immunocytochemistry • Immunofluorescence

psi-iconBackground

PD-L1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) (1). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC (2). PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells (3). This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. PD-L1 also functions as an immune checkpoint protein, and multiple anti-PD-L1 antibodies are currently in phase II and III clinical trials, with one antibody already approved for the treatment of cancer (4).


psi-iconReferences

1) Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
2) Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
3) LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.
4) Aydin AM, Woldu SL, Hutchinson RC, et al. Spotlight on atezolizumab and its potential in the treatment of advanced urothelial bladder cancer.Onco. Targets Ther. 2017;10:1487-502.


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 Price Options

1 Set - $349.00

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Set consists of 20μg per clone.

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