Cat. No.: RF16000

PD-1 Mouse mAb Detection Set (Risk-Free™)

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins.

Each Risk-Free™ monoclonal antibody set consists of several 20μg trial size mAbs with different binding features to help you find the best clone for your specific application. Go to set contents.

ProSci Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications.


Flow cytometry analysis of PD-1 in over expressing HEK293 cells using (A) RF16003, (B) RF16004, (C) RF16005, (D) RF16006, and (E) mouse IgG at 1 μg/ml.  Blue:  untransfected HEK293 cells.  Yellow: PD-1 over expressing HEK293 cells.

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Flow cytometry analysis of PD-1 in over expressing HEK293 cells using (A) RF16003, (B) RF16004, (C) RF16005, (D) RF16006, and (E) mouse IgG at 1 μg/ml.  Blue:  untransfected HEK293 cells.  Yellow: PD-1 over expressing HEK293 cells. See high quality image.

Western blot analysis of PD-1 in overexpressing 293 cells using RF16001, RF16002, and RF16003 antibody at 1, 0.5, and 0.25 μg/ml, respectively

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Western blot analysis of PD-1 in overexpressing 293 cells using RF16001, RF16002, and RF16003 antibody at 1, 0.5, and 0.25 μg/ml, respectively. See high quality image.

A sandwich ELISA was performed using the anti-PD-1 mAbs RF16006 as the capture antibody for the PD-1 extracellular domain antigen with Biotin-labeled Risk-Free anti-PD-1 mAb RF16003 as the detection antibody.

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A sandwich ELISA was performed using the anti-PD-1 mAbs RF16006 as the capture antibody for the PD-1 extracellular domain antigen with Biotin-labeled Risk-Free anti-PD-1 mAb RF16003 as the detection antibody. See high quality image.

Immunofluorescence of PD-1 in over expressing HEK293 cells using (A) RF16001, (B) RF16002, (C) RF16003, (D) RF16004, (E) RF16005, (F) RF16006, and (G) control mouse IgG antibody at 20 μg/ml.

(Click image to expand)
Immunofluorescence of PD-1 in over expressing HEK293 cells using (A) RF16001, (B) RF16002, (C) RF16003, (D) RF16004, (E) RF16005, (F) RF16006, and (G) control mouse IgG antibody at 20 μg/ml. See high quality image.

Immunohistochemistry of PD-1 in human lymph node tissue using (A) RF16001, (B) RF16002, (C) RF16003, (D) RF16004, (E) RF16005, and (F) control mouse IgG antibody at 5 μg/ml

(Click image to expand)
Immunofluorescence of PD-1 in human lymph node tissue using (A) RF16001, (B) RF16002, (C) RF16003, (D) RF16004, (E) RF16005, (F) RF16006, and (G) control mouse IgG antibody at 20 μg/ml. See high quality image.

Immunocytochemistry of PD-1 in over expressing HEK293 cells using (A) RF16001, (B) RF16002,  (C) RF16003, (D) RF16004, (E) RF16005, (F) RF16006, and (G) control mouse IgG antibody at 10 μg/ml.

(Click image to expand)
Immunocytochemistry of PD-1 in over expressing HEK293 cells using (A) RF16001, (B) RF16002,  (C) RF16003, (D) RF16004, (E) RF16005, (F) RF16006, and (G) control mouse IgG antibody at 10 μg/ml. See high quality image.

Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA with RF16001, RF16002, RF16003, RF16004, and RF16005 abs at decreasing concentrations

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Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA with RF16001, RF16002, RF16003, RF16004, RF16005, and RF16006 antibodies at decreasing concentrations. See high quality image.

PD-1 Mouse Monoclonal Set Contents

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PD-1 Mouse Monoclonal Set Contents. See high quality image.

psi-iconSpecifications

NamePD-1 Detection Set (Risk-Free™)
Alternate Names PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1
Host Species Mouse
Tested Applications ELISA, Flow, ICC, IF, IHC-P, WB
Immunogen Mouse monoclonal PD-1 antibodies were raised against the extracellular domain of human PD-1.
Size 20 μg per clone

psi-iconProperties

Clonality Monoclonal
Isotype IgG1
Conjugate Unconjugated
Physical State Liquid
Buffer PBS containing 0.02% sodium azide.
Concentration 1 mg/mL
Storage Conditions Antibodies can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconSet Contents

Hover over application title for rating comparison image.

= Negative = Fair   = Average    = Good     = Excellent= Best Application
Cat. No.ELISAELISA Titration Curve Analysis
ELISA Titration Curve Analysis
WBWestern Blot in HEK293 Cells
Western Blot in HEK293 Cells
ICCICC in HEK293 Cells
ICC in HEK293 Cells
IFIF in HEK293 Cells
IF in HEK293 Cells
IHCIHC in Human Lymph Node Tissue
IHC in Human Lymph Node Tissue
FlowFlow Cytometry in HEK293 Cells
Flow Cytometry in HEK293 Cells
RF16001                   
RF16002                  
RF16003               
RF16004                
RF16005             
RF16006                   

psi-iconProduct Specific Information

ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vitro studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. These antibodies have the following advantages:

  • Antibodies are developed against mammalian-expressed, post-translationally modified recombinant proteins.
  • Multiple antibodies per target allows the user to choose the best antibody for their application
  • Available individually or as a set
  • Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications:
    • • ELISA• Flow Cytometry• Immunoblot • Immunohistochemistry
      • Immunocytochemistry • Immunofluorescence

psi-iconBackground

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins.


psi-iconReferences

1) Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
2) Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
3) Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8.
4) Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51.


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 Price Options

1 Set - $349.00

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Set consists of 20μg per clone.

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