Cat. No.: RF16060

LIGHT Mouse mAb Detection Set (Risk-Free™)

LIGHT Antibody: LIGHT, also known as Tumor Necrosis Factor Superfamily member 14 (TNFSF14), is a co-stimulatory molecule that can regulate T-cell activation (1) and has recently been identified as an immune checkpoint protein. LIGHT binds to two different receptors, Herpes Virus Entry Mediator (HVEM) and Lymphotoxin beta Receptor (LTβR). While LIGHT binding to HVEM delivers a co-stimulatory signal to T cells (1), LIGHT binding to LTβR is critical for the formation of lymphoid structures which can stimulate T cell infiltration and activation of a tumor microenvironment, leading to rapid T cell-mediated tissue destruction (2). It has been shown that targeted delivery of LIGHT to tumors, thereby causing the T cell infiltration of the tumor, can enhance the response of the PD-1/PD-L1 checkpoint blockade anti-cancer therapy (3), suggesting that LIGHT may become a potent tool in anti-cancer treatment.

Each Risk-Free™ monoclonal antibody set consists of several 20μg trial size mAbs with different binding features to help you find the best clone for your specific application. Go to set contents.

ProSci Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications.


Flow cytometry analysis of LIGHT overexpressing HEK293 cells using (A) RF16061, (B) RF16062, (C) RF16063, (D) RF16064, and (E) control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: LIGHT overexpressing HEK293 cells.

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Flow cytometry analysis of LIGHT overexpressing HEK293 cells using (A) RF16061, (B) RF16062, (C) RF16063, (D) RF16064, and (E) control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: LIGHT overexpressing HEK293 cells.See high quality image.

Western blot analysis of LIGHT in overexpressing HEK293 cells using RF16061, RF16062, RF16063, and RF16064 antibody at 0.5 and 1 μg/ml, respectively.

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Western blot analysis of LIGHT in overexpressing HEK293 cells using RF16061, RF16062, RF16063, and RF16064 antibody at 0.5 and 1 μg/ml, respectively. See high quality image.

Immunofluorescence of LIGHT in overexpressing HEK293 cells using (A) RF16061, (B) RF16062, (C) RF16063, (D) RF16064, and (E) control mouse IgG antibody at 2 μg/ml.

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Immunofluorescence of LIGHT in overexpressing HEK293 cells using (A) RF16061, (B) RF16062, (C) RF16063, (D) RF16064, and (E) control mouse IgG antibody at 2 μg/ml. See high quality image.

Immunofluorescence of LIGHT in human lymphoma tissue using using (A) RF16061, (B) RF16062, (C) RF16063, (D) RF16064, and (E) control mouse IgG antibody at 20 μg/ml.

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Immunofluorescence of LIGHT in human lymphoma tissue using using (A) RF16061, (B) RF16062, (C) RF16063, (D) RF16064, and (E) control mouse IgG antibody at 20 μg/ml. See high quality image.

Immunohistochemistry of LIGHT in human lymphoma tissue using using (A) RF16061, (B) RF16062, (C) RF16063, (D) RF16064, and (E) control mouse IgG antibody at 5 μg/ml.

(Click image to expand)
Immunohistochemistry of LIGHT in human lymphoma tissue using using (A) RF16061, (B) RF16062, (C) RF16063, (D) RF16064, and (E) control mouse IgG antibody at 5 μg/ml. See high quality image.

Immunocytochemistry of LIGHT in overexpressing HEK293 cells using (A) RF16061, (B) RF16062, (C) RF16063, (D) RF16064, and (E) control mouse IgG antibody at 1 μg/ml

(Click image to expand)
Immunocytochemistry of LIGHT in overexpressing HEK293 cells using (A) RF16061, (B) RF16062, (C) RF16063, (D) RF16064, and (E) control mouse IgG antibody at 1 μg/ml. See high quality image.

Titration curve analysis of LIGHT mAbs to detect recombinant LIGHT in ELISA with RF16061, RF16062, RF16063, and RF16064 abs at decreasing concentrations.

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Titration curve analysis of LIGHT mAbs to detect recombinant LIGHT in ELISA with RF16061, RF16062, RF16063, and RF16064 abs at decreasing concentrations. See high quality image.

LIGHT Mouse Monoclonal Set Contents	  ]

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LIGHT Mouse Monoclonal Set Contents.

ProSci IconSpecifications

NameLIGHT Detection Set (Risk-Free™)
Alternate Names LIGHT Antibody: TNFSF14, LTg, CD258, HVEML, HVEM ligand
Host Species Mouse
Tested Applications ELISA, Flow, ICC, IF, IHC-P, WB
Immunogen LIGHT antibody was raised against the extracellular domain of human LIGHT.
Size 20 μg per clone

ProSci IconProperties

Clonality Monoclonal
Isotype IgG2b
Conjugate Unconjugated
Physical State Liquid
Buffer LIGHT Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration 1 mg/mL
Storage Conditions Antibodies can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Set Contents

Hover over application title for rating comparison image.

= Negative = Fair   = Average    = Good     = Excellent= Best Application
Cat. No.ELISAELISA Titration Curve Analysis
ELISA Titration Curve Analysis
WBWestern Blot in HEK293 Cells
Western Blot in HEK293 Cells
ICCICC in HEK293 Cells
ICC in HEK293 Cells
IF
IF in HEK293 Cells
IHCIHC in Human Lymphoma
IHC in Human Lymphoma
FlowFlow Cytometry in HEK293 Cells
Flow Cytometry in HEK293 Cells
RF16061                     
RF16062                      
RF16063                       
RF16064                     

Product Specific Information

ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vitro studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. These antibodies have the following advantages:

  • Antibodies are developed against mammalian-expressed, post-translationally modified recombinant proteins.
  • Multiple antibodies per target allows the user to choose the best antibody for their application
  • Available individually or as a set
  • Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications:
    • • ELISA• Flow Cytometry• Immunoblot • Immunohistochemistry
      • Immunocytochemistry • Immunofluorescence

Background

LIGHT Antibody: LIGHT, also known as Tumor Necrosis Factor Superfamily member 14 (TNFSF14), is a co-stimulatory molecule that can regulate T-cell activation (1) and has recently been identified as an immune checkpoint protein. LIGHT binds to two different receptors, Herpes Virus Entry Mediator (HVEM) and Lymphotoxin beta Receptor (LTβR). While LIGHT binding to HVEM delivers a co-stimulatory signal to T cells (1), LIGHT binding to LTβR is critical for the formation of lymphoid structures which can stimulate T cell infiltration and activation of a tumor microenvironment, leading to rapid T cell-mediated tissue destruction (2). It has been shown that targeted delivery of LIGHT to tumors, thereby causing the T cell infiltration of the tumor, can enhance the response of the PD-1/PD-L1 checkpoint blockade anti-cancer therapy (3), suggesting that LIGHT may become a potent tool in anti-cancer treatment.


References

1) Wang Y, Zhu M, Miller M, et al. Immunoregulation by tumor necrosis factor superfamily member LIGHT. Immunological Reviews 2009; 229:232–43.
2) Lee Y, Chin RK, Christiansen P, et al. Recruitment and activation of naive T cells in the islets by lymphotoxin beta receptor-dependent tertiary lymphoid structure. Immunity 2006; 25:499-509.
3) Tang H, Wang Y, Chlewicki LK, et al. Facilitating T cell infiltration in tumor microenvironment overcomes resistance to PD-L1 blockade. Cancer Cell 2016; 29:285-296.


ANTIBODIES FOR RESEARCH USE ONLY.

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1 Set - $249.00

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Set consists of 20μg per clone.

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