Cat. No.:RF16080

LAG-3 Mouse mAb Detection Set (Risk-Free™)

LAG-3 Antibody: The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5).

Each Risk-Free™ monoclonal antibody set consists of several 20μg trial size mAbs with different binding features to help you find the best clone for your specific application. Go to set contents.

ProSci Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications.


Flow cytometry analysis of LAG-3 over expressing HEK293 cells using (A) RF16082, (B) RF16083, (C) RF16084, (D) RF16086, (E) RF16087, (F) RF16088, (G) RF16089, and (H) control mouse IgG antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells

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Flow cytometry analysis of LAG-3 over expressing HEK293 cells using (A) RF16082, (B) RF16083, (C) RF16084, (D) RF16086, (E) RF16087, (F) RF16088, (G) RF16089, and (H) control mouse IgG antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells. See high quality image.

Western blot analysis of LAG-3 in over expressing HEK293 cells using RF16082, RF16088, and RF16089 antibodies at (A) 0.25 μg/ml , (B) 0.5 μg/ml, and (C) 1 μg/ml

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Western blot analysis of LAG-3 in over expressing HEK293 cells using RF16082, RF16088, and RF16089 antibodies at (A) 0.25 μg/ml , (B) 0.5 μg/ml, and (C) 1 μg/ml. See high quality image.

Immunofluorescence of LAG-3 in over expressing HEK293 cells using (A) RF16082,(B) RF16083, (C) RF16084, (D) RF16086, (E) RF16087, (F) RF16088, (G) RF16089, and (H) control mouse IgG antibody at 2 μg/ml.

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Immunofluorescence of LAG-3 in over expressing HEK293 cells using (A) RF16082, (B) RF16083, (C) RF16084, (D) RF16086, (E) RF16087, (F) RF16088, (G) RF16089, and (H) control mouse IgG antibody at 2 μg/ml. See high quality image.

Immunofluorescence of LAG-3 in human spleen tissue using (A) RF16082, (B) RF16084, (C) RF16086, (D) RF16087, (E) RF16088, (F) RF16089, and (G) control mouse IgG antibody at 10 μg/ml

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Immunofluorescence of LAG-3 in human spleen tissue using (A) RF16082, (B) RF16084, (C) RF16086, (D) RF16087, (E) RF16088, (F) RF16089, and (G) control mouse IgG antibody at 10 μg/ml. See high quality image.

Immunocytochemistry of LAG-3 in over expressing HEK293 cells using (A) RF16082, (B) RF16083, (C) RF16084, (D) RF16086, (E) RF16087, (F) RF16088, (G) RF16089, and (H) control mouse IgG antibody at 1 μg/ml

(Click image to expand)
Immunocytochemistry of LAG-3 in over expressing HEK293 cells using (A) RF16082, (B) RF16083, (C) RF16084, (D) RF16086, (E) RF16087, (F) RF16088, (G) RF16089, and (H) control mouse IgG antibody at 1 μg/ml. See high quality image.

Immunohistochemistry of LAG-3 in human lymphoma tissue using (A) RF16082, (B) RF16084, (C) RF16086, (D) RF16087, (E) RF16088, (F) RF16089, and (G) control mouse IgG antibody at 5 μg/ml

(Click image to expand)
Immunohistochemistry of LAG-3 in human lymphoma tissue using (A) RF16082, (B) RF16084, (C) RF16086, (D) RF16087, (E) RF16088, (F) RF16089, and (G) control mouse IgG antibody at 5 μg/ml. See high quality image.

Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with RF16082, RF16083, RF16084, RF16086, RF16087, RF16088, and RF16089 antibodies at decreasing concentrations.

(Click image to expand)
Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with RF16082, RF16083, RF16084, RF16086, RF16087, RF16088, and RF16089 antibodies at decreasing concentrations. See high quality image.

LAG-3 Mouse Monoclonal Set Contents

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LAG-3 Mouse Monoclonal Set Contents.

ProSci IconSpecifications

NameLAG-3 Detection Set (Risk-Free™)
Alternate Names LAG3 Antibody: lymphocyte activating 3, LAG-3, CD223
Host Species Mouse
Tested Applications ELISA, Flow, ICC, IF, IHC-P, WB
Immunogen Mouse monoclonal LAG-3 antibodies were raised against the extracellular domain of human LAG-3.
Size 20 μg per clone

Properties

Clonality Monoclonal
Isotype IgG1,IgG2a
Conjugate Unconjugated
Physical State Liquid
Buffer PBS containing 0.02% sodium azide.
Concentration 1 mg/mL
Storage Conditions Antibodies can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ProSci IconSet Contents

Hover over application title for rating comparison image.

<<
= Negative = Fair   = Average    = Good     = Excellent= Best Application
Cat. No.ELISA
ELISA Titration Curve Analysis
WB
Western Blot in HEK293 Cells
ICC
ICC in HEK293 Cells
IF
IF in HEK293 Cells
IHC
IHC in Human Tonsil Tissue
Flow
Flow Cytometry in HEK293 Cells
RF16082                       
RF16083                  
RF16084                    
RF16086                  
RF16087                  
RF16088                    
RF16089                    

ProSci IconProduct Specific Information

ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vitro studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. These antibodies have the following advantages:

  • Antibodies are developed against mammalian-expressed, post-translationally modified recombinant proteins.
  • Multiple antibodies per target allows the user to choose the best antibody for their application
  • Available individually or as a set
  • Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications:
    • • ELISA• Flow Cytometry• Immunoblot • Immunohistochemistry
      • Immunocytochemistry • Immunofluorescence

ProSci IconBackground

LAG-3 Antibody: The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5).


ProSci IconReferences

1) Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216–21.
2) Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619–22.
3) Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885–91.
4) Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970–9.


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 Price Options

1 Set - $399.00

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Set consists of 20μg per clone.

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