Cat. No.: RF16010

CTLA-4 Mouse mAb Detection Set (Risk-Free™)

The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells (1,2). Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs) (3). Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases (4). CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treatment of cancer (5).

Each Risk-Free™ monoclonal antibody set consists of several 20μg trial size mAbs with different binding features to help you find the best clone for your specific application. Go to set contents.

ProSci Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications.


Western blot analysis of CTLA-4 in overexpressing 293 cells using RF16011, RF16012, and RF16013 antibody at 1, 0.5, and 0.25 μg/ml, respectively

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Western blot analysis of CTLA-4 in overexpressing 293 cells using RF16011, RF16012, and RF16013 antibody at 1, 0.5, and 0.25 μg/ml, respectively. See high quality image.

A sandwich ELISA was performed using the anti-CTLA-4 mAbs RF16011, RF16012, and RF16013 as the capture antibodies for the CTLA-4 extracellular domain antigen with HRP-labeled Risk-Free anti-CTLA-4 mAbs as the detection antibodies

(Click image to expand)
A sandwich ELISA was performed using the anti-CTLA-4 mAbs RF16011, RF16012, and RF16013 as the capture antibodies for the CTLA-4 extracellular domain antigen with HRP-labeled Risk-Free anti-CTLA-4 mAbs as the detection antibodies.See high quality image.

Immunofluorescence of CTLA-4 in overexpressing 293 cells using (A) RF16011, (B) RF16012, (C) RF16013, and (D) control mouse IgG antibody at 20 μg/ml

(Click image to expand)
Immunofluorescence of CTLA-4 in overexpressing 293 cells using (A) RF16011, (B) RF16012, (C) RF16013, and (D) control mouse IgG antibody at 20 μg/ml. See high quality image.

Immunofluorescence of CTLA-4 in human tonsil tissue using (A) RF16011, (B) RF16012, (C) RF16013, and (D) control mouse IgG antibody at at 20 μg/ml

(Click image to expand)
Immunofluorescence of CTLA-4 in human tonsil tissue using (A) RF16011, (B) RF16012, (C) RF16013, and (D) control mouse IgG antibody at at 20 μg/ml. See high quality image.

Immunohistochemistry of CTLA-4 in human lymph node tissue using (A) RF16011, (B) RF16012, (C) RF16013 and (D) control mouse IgG antibody at 5 μg/ml

(Click image to expand)
Immunohistochemistry of CTLA-4 in human lymph node tissue using (A) RF16011, (B) RF16012, (C) RF16013 and (D) control mouse IgG antibody at 5 μg/ml. See high quality image.

Immunohistochemistry of CTLA-4 in human tonsil tissue using (A) RF16011, (B) RF16012, (C) RF16013 and (D) control mouse IgG antibody at 5 μg/ml

(Click image to expand)
Immunohistochemistry of CTLA-4 in human tonsil tissue using (A) RF16011, (B) RF16012, (C) RF16013 and (D) control mouse IgG antibody at 5 μg/ml. See high quality image.

Immunocytochemistry of CTLA-4 in overexpressing 293 cells using (A) RF16011, (B) RF16012, (C) RF16013, and (D) control mouse IgG antibody at 5 μg/ml

(Click image to expand)
Immunocytochemistry of CTLA-4 in overexpressing 293 cells using (A) RF16011, (B) RF16012, (C) RF16013, and (D) control mouse IgG antibody at 5 μg/ml. See high quality image.

Titration curve analysis of CTLA-4 mAbs to detect recombinant CTLA-4 in ELISA with RF16011, RF16012, and RF16013 antibodies at decreasing concentrations

(Click image to expand)
Titration curve analysis of CTLA-4 mAbs to detect recombinant CTLA-4 in ELISA with RF16011, RF16012, and RF16013 antibodies at decreasing concentrations. See high quality image.

psi-iconSpecifications

NameCTLA-4 Detection Set (Risk-Free™)
Alternate Names CTLA-4 Antibody: CTL4, cytotoxic T-lymphocyte associated protein 4, CD, GSE, GRD4, ALPS5, CD152, IDDM12, CELIAC3
Host Species Mouse
Tested Applications ELISA, ICC, IF, IHC-P, WB
Immunogen Mouse monoclonal CTLA-4 antibodies were raised against the extracellular domain of human CTLA-4.
Size 20 μg per clone

ProSci IconProperties

Clonality Monoclonal
Isotype IgG1
Conjugate Unconjugated
Physical State Liquid
Buffer PBS containing 0.02% sodium azide.
Concentration 1 mg/mL
Storage Conditions Antibodies can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ProSci IconSet Contents

Hover over application title for rating comparison image.

= Negative = Fair   = Average    = Good     = Excellent= Best Application
Cat. No.ELISAELISA Titration Curve Analysis
ELISA Titration Curve Analysis
WB>Western Blot in HEK293 Cells
Western Blot in HEK293 Cells
ICCICC in HEK293 Cells
ICC in HEK293 Cells
IFIF in HEK293 Cells
IF in HEK293 Cells
IHCIHC in Human Lymph Node Tissue
IHC in Human Lymph Node Tissue
Flow
RF16011                      
RF16012                 
RF16013                  

ProSci IconProduct Specific Information

ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vitro studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. These antibodies have the following advantages:

  • Antibodies are developed against mammalian-expressed, post-translationally modified recombinant proteins.
  • Multiple antibodies per target allows the user to choose the best antibody for their application
  • Available individually or as a set
  • Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications:
    • • ELISA• Flow Cytometry• Immunoblot • Immunohistochemistry
      • Immunocytochemistry • Immunofluorescence

ProSci IconBackground

The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells (1,2). Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs) (3). Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases (4). CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treatment of cancer (5).


ProSci IconReferences

1) Brunet JF, Denizot F, Luciani MF, et al. A new member of the immunoglobulin superfamily--CTLA-4 . Nature 1987; 328:267–70.
2) Walunas TL, Lenschow DJ, Bakker CY, et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1994; 1:405–13.
3) Harding FA, McArthur JG, Gross JA, et al. CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature 1992; 356:607–9.
4) Romo-Tena J, Gomez-Martin D, and Alcocer-Verela J. CTLA-4 and autoimmunity: new insights into the dual regulator of tolerance. Autoimmun. Rev. 2013; 12:117.


ANTIBODIES FOR RESEARCH USE ONLY.

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 Price Options

1 Set - $199.00

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Set consists of 20μg per clone.

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