Cat. No.:RF16040

CD80 Mouse mAb Detection Set (Risk-Free™)

CD80 Antibody: CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).

Each Risk-Free™ monoclonal antibody set consists of several 20μg trial size mAbs with different binding features to help you find the best clone for your specific application. Go to set contents.

ProSci Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications.


Flow cytometry analysis of CD80 overexpressing HEK293 cells using (A) RF16041, (B) RF16042, (C) RF16044, (D) RF16045, (E) RF16046, and (F) control mouse IgG antibody at 10 μg/ml.  Blue:  Untransfected HEK293 cells.  Yellow:  CD80 overexpressing HEK293 cells.

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Flow cytometry analysis of CD80 overexpressing HEK293 cells using (A) RF16041, (B) RF16042, (C) RF16044, (D) RF16045, (E) RF16046, and (F) control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: CD80 overexpressing HEK293 cells. See high quality image.

Western blot analysis of CD80 in overexpressing HEK293 cells using RF16045 and RF16046 antibody at 0.25 and 0.5 μg/ml

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Western blot analysis of CD80 in overexpressing HEK293 cells using RF16045 and RF16046 antibody at 0.25 and 0.5 μg/ml, respectively. See high quality image.

Immunofluorescence of CD80 in overexpressing HEK293 cells using (A) polyclonal CD80 antibody, (B) RF16041, (C) RF16042, (D) RF16043, (E) RF16044, (F) RF16045, (G) RF16046 and (H) control mouse IgG antibody at 2 μg/ml.

(Click image to expand)
Immunofluorescence of CD80 in overexpressing HEK293 cells using (A) polyclonal CD80 antibody, (B) RF16041, (C) RF16042, (D) RF16043, (E) RF16044, (F) RF16045, (G) RF16046 and (H) control mouse IgG antibody at 2 μg/ml. See high quality image.

Immunofluorescence of CD80 in human stomach carcinoma tissue using (A) polyclonal CD80 antibody, (B) RF16041, (C) RF16042, (D) RF16043, (E) RF16044, (F) RF16045, (G) RF16046 and (H) control mouse IgG antibody at 20 μg/ml.

(Click image to expand)
Immunofluorescence of CD80 in human stomach carcinoma tissue using (A) polyclonal CD80 antibody, (B) RF16041, (C) RF16042, (D) RF16043, (E) RF16044, (F) RF16045, (G) RF16046 and (H) control mouse IgG antibody at 20 μg/ml. See high quality image.

Immunofluorescence of CD80 in human stomach carcinoma tissue using (A) polyclonal CD80 antibody, (B) RF16041, (C) RF16042, (D) RF16043, (E) RF16044, (F) RF16045, (G) RF16046 and (H) control mouse IgG antibody at 20 μg/ml

(Click image to expand)
Immunofluorescence of CD80 in human stomach carcinoma tissue using (A) polyclonal CD80 antibody, (B) RF16041, (C) RF16042, (D) RF16043, (E) RF16044, (F) RF16045, (G) RF16046 and (H) control mouse IgG antibody at 20 μg/ml. See high quality image.

Immunohistochemistry of CD80 in human stomach carcinoma tissue using (A) polyclonal CD80 antibody at 1 μg/ml, (B) RF16041, (C) RF16042, (D) RF16043, (E) RF16044, (F) RF16045, (G) RF16046 and (H) control mouse IgG antibody at 5 μg/ml

(Click image to expand)
Immunohistochemistry of CD80 in human stomach carcinoma tissue using (A) polyclonal CD80 antibody at 1 μg/ml, (B) RF16041, (C) RF16042, (D) RF16043, (E) RF16044, (F) RF16045, (G) RF16046 and (H) control mouse IgG antibody at 5 μg/ml. See high quality image.

Immunohistochemistry of CD80 in tonsil tissue using (A) polyclonal CD80 antibody at 1 μg/ml, (B) RF16041, (C) RF16042, (D) RF16043, (E) RF16044, (F) RF16045, (G) RF16046 and (H) control mouse IgG antibody at 5 μg/ml

(Click image to expand)
Immunohistochemistry of CD80 in tonsil tissue using (A) polyclonal CD80 antibody at 1 μg/ml, (B) RF16041, (C) RF16042, (D) RF16043, (E) RF16044, (F) RF16045, (G) RF16046 and (H) control mouse IgG antibody at 5 μg/ml. See high quality image.

Immunocytochemistry of CD80 in overexpressing HEK293 cells using (A) polyclonal CD80 antibody, (B) RF16041, (C) RF16042, (D) RF16043, (E) RF16044, (F) RF16045, (G) RF16046 and (H) control mouse IgG antibody at 1 μg/ml

(Click image to expand)
Immunocytochemistry of CD80 in overexpressing HEK293 cells using (A) polyclonal CD80 antibody, (B) RF16041, (C) RF16042, (D) RF16043, (E) RF16044, (F) RF16045, (G) RF16046 and (H) control mouse IgG antibody at 1 μg/ml. See high quality image.

Titration curve analysis of CD80 mAbs to detect recombinant CD80 in ELISA with RF16041, RF16042, RF16043, RF16044, RF16045, and RF16046 abs at decreasing concentrations

(Click image to expand)
Titration curve analysis of CD80 mAbs to detect recombinant CD80 in ELISA with RF16041, RF16042, RF16043, RF16044, RF16045, and RF16046 abs at decreasing concentrations. See high quality image.

ProSci IconSpecifications

NameCD80 Detection Set (Risk-Free™)
Alternate Names CD80 Antibody: CD80 molecule, B7, BB1, B7-1, B7.1, LAB7, CD28LG, CD28LG1
Host Species Mouse
Tested Applications ELISA, Flow, ICC, IF, IHC-P, WB
Immunogen Mouse monoclonal CD80 antibodies were raised against the extracellular domain of human CD80.
Size 20 μg per clone

ProSci IconProperties

Clonality Monoclonal
Isotype IgG1
Conjugate Unconjugated
Physical State Liquid
Buffer PBS containing 0.02% sodium azide.
Concentration 1 mg/mL
Storage Conditions Antibodies can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ProSci IconSet Contents

Hover over application title for rating comparison image.

= Negative = Fair   = Average    = Good     = Excellent= Best Application
Cat. No.ELISAELISA Titration Curve Analysis
ELISA Titration Curve Analysis
WBWestern Blot in HEK293 Cells
Western Blot in HEK293 Cells
ICCICC in HEK293 Cells
ICC in HEK293 Cells
IFIF in HEK293 Cells
IF in HEK293 Cells
IHCIHC in Human Tonsil Tissue
IHC in Human Tonsil Tissue
FlowFlow Cytometry in HEK293 Cells
Flow Cytometry in HEK293 Cells
RF16041                     
RF16042                     
RF16043                    
RF16044                     
RF16045                       
RF16046                       

ProSci IconProduct Specific Information

ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vitro studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. These antibodies have the following advantages:

  • Antibodies are developed against mammalian-expressed, post-translationally modified recombinant proteins.
  • Multiple antibodies per target allows the user to choose the best antibody for their application
  • Available individually or as a set
  • Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications:
    • • ELISA• Flow Cytometry• Immunoblot • Immunohistochemistry
      • Immunocytochemistry • Immunofluorescence

ProSci IconBackground

CD80 Antibody: CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).


ProSci IconReferences

1) Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400.
2) Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.


ANTIBODIES FOR RESEARCH USE ONLY.

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1 Set - $349.00

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Set consists of 20μg per clone.

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