Cat. No.: RF16090

B7-H3 Mouse mAb Detection Set (Risk-Free™)

B7-H3 Antibody: CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.

Each Risk-Free™ monoclonal antibody set consists of several 20μg trial size mAbs with different binding features to help you find the best clone for your specific application. Go to set contents.

ProSci Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications.


Flow cytometry analysis of B7-H3 in HEK293 cells using (A) RF16091, (B) RF16092, (C) RF16093, (D) RF16094, and (E) RF16095 at 1 μg/ml.  Blue:  isotype control on HEK293 cells.  Yellow:  B7-H3 mAb on HEK293 cells

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Flow cytometry analysis of B7-H3 in HEK293 cells using (A) RF16091, (B) RF16092, (C) RF16093, (D) RF16094, and (E) RF16095 at 1 μg/ml. Blue: isotype control on HEK293 cells. Yellow: B7-H3 mAb on HEK293 cells. See high quality image.

Western blot analysis of B7-H3 in HEK293 cells using RF16091, RF16092, and RF16093 antibodies at (A) 0.25 μg/ml , (B) 0.5 μg/ml,  and (C) 1 μg/ml

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Western blot analysis of B7-H3 in HEK293 cells using RF16091, RF16092, and RF16093 antibodies at (A) 0.25 μg/ml , (B) 0.5 μg/ml, and (C) 1 μg/ml. See high quality image.

Immunofluorescence of B7-H3 in human colon carcinoma tissue using (A) RF16092, (B) RF16093, (C) RF16094, (D) RF16095, and (E) control mouse IgG antibody at 10 μg/ml

(Click image to expand)
Immunofluorescence of B7-H3 in human colon carcinoma tissue using (A) RF16092, (B) RF16093, (C) RF16094, (D) RF16095, and (E) control mouse IgG antibody at 10 μg/ml. See high quality image.

Immunofluorescence of B7-H3 in HEK293 cells using (A) RF16091, (B) RF16092, (C) RF16093, (D) RF16094, (E) RF16095, and (F) control mouse IgG antibody at 5 μg/ml

(Click image to expand)
Immunofluorescence of B7-H3 in HEK293 cells using (A) RF16091, (B) RF16092, (C) RF16093, (D) RF16094, (E) RF16095, and (F) control mouse IgG antibody at 5 μg/ml. See high quality image.

Immunohistochemistry of B7-H3 in human colon carcinoma tissue using (A) RF16091, (B) RF16092, (C) RF16093, (D) RF16094, (E) RF16095, and (F) control mouse IgG antibody at 2 μg/ml

(Click image to expand)
Immunohistochemistry of B7-H3 in human colon carcinoma tissue using (A) RF16091, (B) RF16092, (C) RF16093, (D) RF16094, (E) RF16095, and (F) control mouse IgG antibody at 2 μg/ml. See high quality image.

Immunocytochemistry of B7-H3 in HEK293 cells using (A) RF16091, (B) RF16092,  (C) RF16093, (D) RF16094, (E) RF16095, and (F) control mouse IgG antibody at 1 μg/ml.

(Click image to expand)
Immunocytochemistry of B7-H3 in HEK293 cells using (A) RF16091, (B) RF16092, (C) RF16093, (D) RF16094, (E) RF16095, and (F) control mouse IgG antibody at 1 μg/ml. See high quality image.

Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA with RF16091, RF16092, RF16093, RF16094, and RF16095 antibodies at decreasing concentrations

(Click image to expand)
Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA with RF16091, RF16092, RF16093, RF16094, and RF16095 antibodies at decreasing concentrations. See high quality image.

(Click image to expand)
B7-H3 Mouse Monoclonal Set Contents.

ProSci IconSpecifications

NameB7-H3 Detection Set (Risk-Free™)
Alternate Names B7-H3 Antibody: CD276 molecule, B7H3, B7RP-2, 4Ig-B7-H3, CD276
Host Species Mouse
Tested Applications ELISA, Flow, ICC, IF, IHC-P, WB
Immunogen B7-H3 antibody was raised against the extracellular domain of human B7-H3.
Size 20 μg per clone

ProSci IconProperties

Clonality Monoclonal
Isotype IgG2b
Conjugate Unconjugated
Physical State Liquid
Buffer B7-H3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration 1 mg/mL
Storage Conditions Antibodies can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ProSci IconSet Contents

Hover over application title for rating comparison image.

= Negative = Fair   = Average    = Good     = Excellent= Best Application
Cat. No.ELISAELISA Titration Curve Analysis
ELISA Titration Curve Analysis
WBWestern Blot in HEK293 Cells
Western Blot in HEK293 Cells
ICCICC in HEK293 Cells
ICC in HEK293 Cells
IFIF in HEK293 Cells
IF in HEK293 Cells
IHCIHC in Human Lymphoma
IHC in Human Lymphoma
FlowFlow Cytometry in HEK293 Cells
Flow Cytometry in HEK293 Cells
RF16091               
RF16092                        
RF16093                        
RF16094                       
RF16095                    

Product Specific Information

ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vitro studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. These antibodies have the following advantages:

  • Antibodies are developed against mammalian-expressed, post-translationally modified recombinant proteins.
  • Multiple antibodies per target allows the user to choose the best antibody for their application
  • Available individually or as a set
  • Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications:
    • • ELISA• Flow Cytometry• Immunoblot • Immunohistochemistry
      • Immunocytochemistry • Immunofluorescence

Background

B7-H3 Antibody: CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.


References

1) Chapoval AI, Ni J, Lau JS, et al. B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production. Nat Immunol 2001; 2:269-74.
2) Sun M, Richards S, Prasad DV, et al. Characterization of mouse and human B7-H3 genes. J Immunol 2002; 168:6294-7.
3) 3) Yi KH and Chen L. Fine tuning the immune response through B7-H3 and B7-H4. Immunol Rev 2009; 229:145-51.
4) Wang L, Kang FB, and Shan BE. B7-H3-mediated tumor immunology: friend or foe?. Int J Cancer 2014; 134:2764-71.


ANTIBODIES FOR RESEARCH USE ONLY.

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 Price Options

1 Set - $299.00

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Set consists of 20μg per clone.

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