Risk-Free™ Immune Checkpoint Antibody Sets

Multiple antibodies for multiple applications

Many antibodies work well in one application but not in others despite detecting the same proteins. The reason is because the target antigen may be denatured or inaccessible depending on the technique or preparation. For example in flow cytometry, the protein of interest is still in its native state on the cell surface while in western blot the protein is completely denatured. Because of this nature of antibodies, scientists must go through searching for and testing multiple antibodies for one application and find that they must repeat the process again for a different application. ProSci has recognized this issue and has released Risk-Free™ Immune Checkpoint Antibody Sets to assist with this issue.

Development of Risk-Free™ Antibody Sets

Each set consists of Risk-Free™ monoclonal antibodies developed with recombinant protein antigens expressed in mammalian cells to best recognize immune checkpoint targets in their most native form. Because of this process of development, Risk-Free™ monoclonal antibodies epitopes binding features have a broad range and work in multiple applications. Rigorously tested in a variety of applications, the best antibodies were carefully selected and paired together to provide broadest range of binding features for scientist to select from. Below are the available targets to choose from.

Features of ProSci's Risk-Free™ mAb Sets

Developed with recombinant proteins expressed in mammalian cells to best recognize the native proteins.

Each set consists of several mAbs with different binding features allowing selection of the best clone.

Risk-Free™ means these mAb sets are rigorously tested and guaranteed to work
for their designated applications.

 

Risk-Free™ Immune Checkpoint Mouse Monoclonal Sets

Product Set	Clones	ELISA	WB	ICC	IF	IHC	Flow	Pairs
PD-1	5
PD-L1	6
PD-L2	5							TBD
CTLA-4	3
CD80	6							TBD
TIGIT	6
LIGHT	4
VISTA	4
LAG-3	7
B7-H3	5
LAG-3	7
TIM-3	7
TMIGD2	3

Risk-Free™ Immune Checkpoint Llama Single Domain Sets

Llama single domain antibodies (sdAbs) offer unique characteristics amenable to many applications. These sdAbs demonstrate low nanomolar binding affinity and can be used for the various applications. Note: LIGHT single domain antibody should be stored in working aliquots at -20 °C, stable for up to one year. As with all antibodies, care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Set	Clones	ELISA	WB	ICC	IF	IHC	Flow	Functionality	Pairs
PD-1	4

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