PD-1 Immune Checkpoint

 

The PD-1 protein is a negative co-stimulatory receptor that can bind two different but related ligands, PD-L1 and PD-L2. Upon binding, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals."


Match Pair Sets

A sandwich ELISA was performed using the anti-PD-1 mAbs RF16001, RF16002, RF16003, RF16004, and RF16005 as the capture antibodies for the PD-1 extracellular domain antigen with HRP-labeled Risk-Free™ anti-PD-1 mAbs as the detection antibodies.

TargetCat. No.CaptureDetectionHostDatasheet
PD-1 Human Matched Antibody Pair #1
*See Capture ELISA Data*PD-1 Matched Pair ELISA Data
RFP-0001 RF16004 RF16003-HRP Mouse - Mouse
PD-1 Human Matched Antibody Pair #2
*See Capture ELISA Data*PD-1 Matched Pair ELISA Data
RFP-0002 RF16005 RF16002-HRP Mouse - Mouse

Risk-Free™ Mouse Monoclonal PD-1 Products

ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are guaranteed to work for their approved applications.
"Hover over research application to see respective image (if available)."

Risk-Free™ Mouse Monoclonal PD-1 Set

ProSci's Risk-Free™ Sets consists of several mAbs with different binding features allowing selection of the best clone.

Product SetSizeContentsDescription
Risk-Free PD-1 Set (RF16000) 20μg / Clone RF16001, RF16002, RF16003, RF16004, RF16005, RF16006 Mouse monoclonal PD-1 antibodies were raised against the extracellular domain of human PD-1.

Risk-Free™ Mouse Monoclonal PD-1 Antibodies

ProSci Risk-Free™ PD-1 antibodies are rigorously tested and guaranteed to work for their designated applications.

= Negative = Fair   = Average    = Good     = Excellent= Best Application
Cat. No.Epitope MapELISAWBICCIFIHCFlowConjugation
RF16001 Host Species: Mouse
Reactivity: Human
PD-1 Epitope Map    PD-1 ELISA Curve
ELISA Titration Curve Analysis
   PD-1 Wester Blot
WB in HEK293 Cells
  PD-1 RF16001 Immunocytochemistry
ICC in HEK293 Cells
  PD-1 RF16001 Immunofluorescence
IF in HEK293 Cells
 PD-1 RF16001 Immunohistochemistry
IHC in Human Lymph Node
   Available
RF16002Host Species: Mouse
Reactivity: Human
Conformation Specific   PD-1 ELISA Curve
ELISA Titration Curve Analysis
  PD-1 Wester Blot
WB in HEK293 Cells
  PD-1 RF16002 Immunocytochemistry
ICC in HEK293 Cells
  PD-1 RF16002 Immunofluorescence
IF in HEK293 Cells
  PD-1 RF16002 Immunohistochemistry
IHC in Human Lymph Node
   Available
RF16003Host Species: Mouse
Reactivity: Human
Conformation Specific   PD-1 ELISA Curve
ELISA Titration Curve Analysis
 PD-1 Wester Blot
WB in HEK293 Cells
  PD-1 RF16003 Immunocytochemistry
ICC in HEK293 Cells
 PD-1 RF16003 Immunofluorescence
IF in HEK293 Cells
  PD-1 RF16003 Immunohistochemistry
IHC in Human Lymph Node
 PD-1 RF16003 Flow Cytometry
Flow in HEK293 Cells
Available
RF16004Host Species: Mouse
Reactivity: Human
Conformation Specific  PD-1 ELISA Curve
ELISA Titration Curve Analysis
     PD-1 RF16004 Immunocytochemistry
ICC in HEK293 Cells
   PD-1 RF16004 Immunofluorescence
IF in HEK293 Cells
  PD-1 RF16004 Immunohistochemistry
IHC in Human Lymph Node
 PD-1 RF16004 Flow Cytometry
Flow in HEK293 Cells
Available
RF16005Host Species: Mouse
Reactivity: Human
Conformation Specific  PD-1 ELISA Curve
ELISA Titration Curve Analysis
    PD-1 RF16005 Immunocytochemistry
ICC in HEK293 Cells
   PD-1 RF16005 Immunofluorescence
IF in HEK293 Cells
  PD-1 RF16005 Immunohistochemistry
IHC in Human Lymph Node
PD-1 RF16005 Flow Cytometry
Flow in HEK293 Cells
Available
RF16006Host Species: Mouse
Reactivity: Human
Conformation Specific   PD-1 ELISA Curve
ELISA Titration Curve Analysis
      PD-1 RF16006 Immunocytochemistry
ICC in HEK293 Cells
    PD-1 RF16006 Immunofluorescence
IF in HEK293 Cells
 PD-1 RF16006 Immunohistochemistry
IHC in Human Lymph Node
  PD-1 RF16005 Flow Cytometry
Flow in HEK293 Cells
Available

Risk-Free™ Single Domain Monoclonal PD-1 Products

Llama single domain antibodies (sdAbs) offer unique characteristics amenable to many applications. These sdAbs demonstrate low nanomolar binding affinity and can be used for the various applications. Note: PD-1 antibody should be stored in working aliquots at -20 °C, stable for up to one year. As with all antibodies, care should be taken to avoid repeated freeze thaw cycles. PD-1 antibodies should not be exposed to prolonged high temperatures.

Risk-Free™ Single Domain Monoclonal PD-1 Set

Product SetSizeContentsDescription
Single Domain Set (SD8600) 20μg / Clone SD8643, SD8645, SD8647, SD8649 Llama single domain monoclonal PD-1 antibodies were raised against a recombinant protein corresponding to amino acids 25 - 167 of human PD-1.

Risk-Free™ Single Domain Monoclonal PD-1 Antibodies

= Not Yet Determined = Negative = Fair   = Average    = Good     = Excellent
Cat. No.CloneELISAWBICCIFIHCFlowFunctionalityDatasheet
SD8643Host Species: Llama
Reactivity: Human
F1-2A8    Single Domain PD-1 ELISA Curve
ELISA Titration Curve Analysis
              PD-1 SD8643 Flow Cytometry
Flow in HEK293 Cells
 
SD8645Host Species: Llama
Reactivity: Human
F4-2D7    Single Domain PD-1 ELISA Curve
ELISA Titration Curve Analysis
              PD-1 SD8645 Flow Cytometry
Flow in HEK293 Cells
  
SD8647Host Species: Llama
Reactivity: Human
F3-4C9    Single Domain PD-1 ELISA Curve
ELISA Titration Curve Analysis
              PD-1 SD8647 Flow Cytometry
Flow in HEK293 Cells
  
SD8649Host Species: Llama
Reactivity: Human
F2-5F4    Single Domain PD-1 ELISA Curve
ELISA Titration Curve Analysis
              PD-1 SD8649 Flow Cytometry
Flow in HEK293 Cells
  

Related Products

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Antigen


Cat. No.Product TypeSpeciesTissue/CellDatasheet
RF16000-01 Recombinant Protein Human HEK293
RF16000-02 Risk-Free PD-1 Mapping Peptide Set Human
 
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Lysates


Cat. No.Product TypeSpeciesTissue/CellDatasheet
RF10001-01 Positive Control (Cell Lysate) Human THP-1
RF10001-03 Positive Control (Tissue Lysate) Human Spleen
RF10001-04 Positive Control (Tissue Lysate) Human Tonsil
RF10001-05 Positive Control (Tissue Lysate) Human Lymph Node
RF10001-02 Negative Control (Cell Lysate) Human HEK293
 
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Slides


Cat. No.Product TypeSpeciesTissue/CellDatasheet
RF10002-01 Positive Control (Cells) Human THP-1
RF10002-03 Positive Control (Tissue) Human Spleen
RF10002-02 Negative Control (Cells) Human HEK293
 
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Conjugates


Cat. No.HRPBiotinCustom
RF16001 Inquire Contact Us
RF16002 Available Inquire Contact Us
RF16003 Available Inquire Contact Us
RF16004 Inquire Contact Us
RF16005 Inquire Contact Us
 

Additional Antibody PD-1 Products

Monoclonal antibodies are highly specific and recognize only one epitope on an antigen which generates consistent performance in quantitative detection applications regardless of production batch. Polyclonal antibodies have high affinity and recognize different epitopes on a specific antigen. Note: These antibodies can be stored at -20˚C and are stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.


Monoclonal PD-1 Products

= Not Yet Determined = Negative = Fair   = Average    = Good     = Excellent
Cat. No.CloneELISAWBICCIFIHCFlowDatasheet
PM-5177Host Species: Mouse
Reactivity: HU, MS, RAT
7A11B1       PD-1 PM-5177 Western Blot
WB in A-20 Cell Lysate
     PD-1 PM-5177 Immunofluorescence
IF in Human Spleen
  PD-1 PM-5177 Immunohistochemistry
IHC in Human Spleen
  
PM-5179Host Species: Mouse
Reactivity: HU, MS, RAT
12A7D7       PD-1 PM-5179 Western Blot
WB in A-20 Cell Lysate
    PD-1 PM-5179 Immunofluorescence
IF in Mouse Thymus
 PD-1 PM-5179 Immunohistochemistry
IHC in Mouse Brain
  

Polyclonal PD-1 Products

= Not Yet Determined = Negative = Fair   = Average    = Good     = Excellent
Cat. No.Antigen RangeELISAWBICCIFIHCFlowDatasheet
4065Host Species: Rabbit
Reactivity: HU, MS
210 - 260        PD-1 4065 Western Blot
WB in THP-1
  PD-1 4065 Immunohistochemistry
ICC in HEK293 Cells
  PD-1 4065 Immunofluorescence
IF in Human Brain
  PD-1 4065 Immunohistochemistry
IHC in Human Brain
  
4067Host Species: Rabbit
Reactivity: HU, MS, RAT
120 - 170       PD-1 4067 Western Blot
WB in THP-1
        PD-1 4067 Immunohistochemistry
IHC in Human Brain
  
46-148Host Species: Goat
Reactivity: HU
220 - 270       PD-1 46-148 Western Blot
WB in Bone Marrow
           

PD-1 Peptide Products

Cat. No.LocationSpeciesDatasheet
4065P 16 amino acids near the carboxy terminus of human PD-1 Human
4067P 16 amino acids near the center of human PD-1 Human
46-148P PDCD1 peptide corresponds to 13 amino acids of PDCD1 Human

Additional PD-1 Information

 

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