CTLA-4 Immune Checkpoint

 

The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells. Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs).


Match Pair Sets

A sandwich ELISA was performed using the anti-CTLA-4 mAbs RF16011, RF16012, RF16013, as the capture antibodies for the CTLA-4 extracellular domain antigen with HRP-labeled Risk-Free anti-CTLA-4 mAbs as the detection antibodies.

TargetCat. No.CaptureDetectionHostDatasheet
CTLA-4 Human Matched Antibody Pair #1
*See Capture ELISA Data*
RFP-0101 RF16011 RF16013-HRP Mouse - Mouse
 

Risk-Free™ Mouse Monoclonal CTLA-4 Products

ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ CTLA-4 antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Risk-Free™ means they are guaranteed to work for their approved applications.
"Hover over research application to see respective image (if available)."

Risk-Free™ Mouse Monoclonal CTLA-4 Set

ProSci's Risk-Free™ Sets consists of several mAbs with different binding features allowing selection of the best clone.

Product SetSizeContentsDescription
Risk-Free CTLA-4 Set (RF16010) 20μg / Clone RF16011, RF16012, RF16013 Mouse monoclonal CTLA-4 antibodies were raised against the extracellular domain of human CTLA-4.

Risk-Free™ Mouse Monoclonal CTLA-4 Antibodies

ProSci Risk-Free™ CTLA-4 antibodies are rigorously tested and guaranteed to work for their designated applications.

= Negative = Fair   = Average    = Good     = Excellent= Best Application
Cat. No.ELISAWBICCIFIHCFlowConjugation
RF16011 Host Species: Mouse
Reactivity: Human
   
ELISA Titration Curve Analysis
   
WB in HEK293 Cells
   
ICC in HEK293 Cells
  
IF in HEK293 Cells
   
IHC in Human Lymph Node
   Available
RF16012Host Species: Mouse
Reactivity: Human
 
ELISA Titration Curve Analysis
  
WB in HEK293 Cells
  
ICC in HEK293 Cells
 
IF in HEK293 Cells
   
IHC in Human Lymph Node
   Available
RF16013Host Species: Mouse
Reactivity: Human
  
ELISA Titration Curve Analysis
  
WB in HEK293 Cells
  
ICC in HEK293 Cells
 
IF in HEK293 Cells
   
IHC in Human Lymph Node
   Available

Risk-Free™ Single Domain Monoclonal CTLA-4 Products

Llama single domain antibodies (sdAbs) offer unique characteristics amenable to many applications. These sdAbs demonstrate low nanomolar binding affinity and can be used for the various applications. Note: CTLA-4 antibody should be stored in working aliquots at -20 °C, stable for up to one year. As with all antibodies, care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Coming Soon

  • Immunization
  • PBMC Isolation
  • Library Construction
  • Validation
  • Release

If you want to be notified when this target will be released, let us know.

Related Products

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Antigen


Cat. No.Product TypeSpeciesTissue/CellDatasheet
RF16010-01 Recombinant Protein Human HEK293
 
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Lysates


Cat. No.Product TypeSpeciesNameDatasheet
RF10001-04 Positive Control (Tissue Lysate) Human Tonsil
RF10001-05 Positive Control (Tissue Lysate) Human Lymph Node
RF10001-02 Negative Control (Cell Lysate) Human HEK293
 
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Slides


No slides currently available.

 
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Conjugates


Cat. No.HRPBiotinCustom
RF16011 Inquire Not Available Contact Us
RF16012 Inquire Not Available Contact Us
RF16013 Available Not Available Contact Us
 

Additional Antibody CTLA-4 Products

Monoclonal antibodies are highly specific and recognize only one epitope on an antigen which generates consistent performance in quantitative detection applications regardless of production batch. Polyclonal antibodies have high affinity and recognize different epitopes on a specific antigen. Note: These antibodies can be stored at -20˚C and are stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.


Monoclonal CTLA-4 Products

Cat. No.NameApplicationsDatasheet
76-842Host Species: Mouse
Reactivity: Human
CD152 Antibody [BNI3] Flow, Func, IHC, IP
76-845Host Species: Mouse
Reactivity: Human
CD152 Antibody [BNI3] (PE) Flow
76-847Host Species: Mouse
Reactivity: Human
CD152 Antibody [BNI3] (APC) Flow
76-849Host Species: Hamster
Reactivity: Mouse
CD152 Antibody [UC10-4F10-11] Flow
76-851Host Species: Hamster
Reactivity: Mouse
CD152 Antibody [BNI3] (PE) Flow
76-853Host Species: Hamster
Reactivity: Mouse
CD152 Antibody [UC10-4F10-11] (PE-Cy7) Flow
76-855Host Species: Hamster
Reactivity: Mouse
CD152 Antibody [UC10-4F10-11] (APC) Flow

Polyclonal CTLA-4 Products

If you are interested in the polyclonal development
of this target, let us know.


CTLA-4 Recombinant Protein Products

Cat. No.SequenceFusion TagSpeciesTissue/CellDatasheet
96-215 Biotinylated form of Recombinant Protein L His Tag Cynomolgus Monkey HEK293
96-216 Pro Val 77 - Leu 233 C-Fc Tag Cynomolgus Monkey HEK293
96-219 Ala 37 - Ser 160 C-Fc Tag Human HEK293
96-220 Ala 37 - Phe 162 C-Twin Strep Tag Human HEK293

Additional CTLA-4 Information

 

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