IL-9 Antibody Cat. No.: 7495

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: Anti-IL-9 antibody (7495) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IL-9.

The immunogen is located within the last 50 amino acids of IL-9.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 1-4 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: IL-9 antibody is human specific. IL-9 antibody is predicted to no cross-react with IL-7.
POSITIVE CONTROL:1) Cat. No. 1306 - Human Spleen Tissue Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
 3) Cat. No. 1201 - HeLa Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 16kD

Observed: 22 kD (Post-modification: 4 N-linked glycosylations)
psi-iconAdvanced Validation
VALIDATION:

Recombinant Protein Test (Figure 2): Anti-IL-9 antibodies (7495) detected human IL-9 recombinant protein at different concentrations.

ISOFORMS: Human IL-9 has one isoform (144aa, 16kD). Mouse IL-9 has one isoform (144aa, 16kD) and Rat IL-9 also has one isoform (144aa, 16kD). 7495 can detect human isoform, but cannot detect mouse and rat.

psi-iconProperties
PURIFICATION:IL-9 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:IL-9 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:IL9
ALTERNATE NAMES:IL-9 Antibody : P40, HP40, IL-9, Interleukin-9, Cytokine P40
ACCESSION NO.:NP_000581
PROTEIN GI NO.:10834980
GENE ID:3578
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Interleukin 9 (IL-9) is a cytokine secreted by TH2 lymphocytes that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation and prevents apoptosis (1,2). It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins (3). The IL-9 gene has been identified as a candidate gene for asthma (4). Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness (5).
REFERENCES:1) Renauld et al. Cytokine 1990; 2:9-12.
2) Tete et al. J. Biol. Homeost. Agents 2012; 26:319-26.
3) Knoops and Renauld. Growth Factors 2004; 22:207-15.
4) Nicolaides et al. Proc. Natl. Acad. Sci. USA 1997; 94:13175-80.

ANTIBODIES FOR RESEARCH USE ONLY.

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