The ED50 was determined by its ability to intensify the IL-6 induced growth inhibition of murine M1 cells is ≤ 5.0 ng/ml, in the presence of 20 ng/ml of rhIL-6.
Properties
PURITY:
≥ 98% by SDS-PAGE gel and HPLC analyses.
PHYSICAL STATE:
Lyophilized
STORAGE CONDITIONS:
The recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing the recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Additional Info
ALTERNATE NAMES:
soluble IL-6 receptor alpha, B cell stimulatory factor-2, CD126
OFFICIAL SYMBOL:
IL6R
GENE ID:
3570
Background and References
BACKGROUND:
IL-6 mediates its biological effects through the type I IL-6 receptor system that consists of two chains, IL-6Rα and gp130. While the IL-6Rα chain is the binding component specific to IL-6, the gp130 chain only transmits signals of IL-6 when bound to IL-6Rα. The gp130 can also transmit signals from LIF, OSM, CNTF, IL-11 and CT-1 in conjunction with other receptor subunits. The low-affinity binding site for IL-6 is composed of IL-6Rα alone. IL-6Rα is expressed in a wide range of cells, including T cells, fibroblasts and macrophages. Soluble IL-6Rα, which consists of only the extracellular domain of the IL-6Rα chain, acts as an agonist of IL-6 activity at low concentrations. Recombinant Human sIL-6Rα is a 37.6 kDa protein consisting of the extracellular domain of the IL-6Rα chain (339 amino acid residues).
Specifications
