Datasheet

IL-37 Antibody
CATALOG NUMBER: 7499

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:IL-37 antibody can be used for detection of IL-37 by Western blot at 1 - 2 μg/ml.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1302 - Human Lung Tissue Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 24 kDa

Observed: 23kDa
SPECIFICITY:IL-37 antibody is human specific. Multiple isoforms of IL-37 are known to exist; this antibody will only detect the largest isoform. IL-37 antibody is predicted to not cross-react with IL-36.
IMMUNOGEN:IL-37 antibody was raised against a 17 amino acid peptide near the amino terminus of human IL-37.

The immunogen is located within amino acids 30 - 80 of IL-37 .
HOST SPECIES:Rabbit

Properties

PURIFICATION:IL-37 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IL-37 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-37 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IL-37 Antibody : FIL1, FIL1Z, IL-1H, IL-37, IL1F7, IL1H4, IL-1F7, IL-1H4, IL1RP1, IL-1RP1, FIL1(ZETA), Interleukin-37, FIL1 zeta
ACCESSION NO.:NP_055254
PROTEIN GI NO.:20127524
OFFICIAL SYMBOL:IL37
GENE ID:27178

Background

BACKGROUND:Interleukin 37 (IL-37) is a member of the interleukin 1 cytokine family (reviewed in 1). It can bind to, and may be a ligand for interleukin 18 receptor (IL18R1/IL-1Rrp). IL-37 also binds to interleukin 18 binding protein (IL18BP), an inhibitory binding protein of interleukin 18 (IL18), and subsequently forms a complex with IL18 receptor beta subunit, and through which it inhibits the activity of IL18 (1). IL-37 can also interact with Smad3 and expression of IL-37 in macrophages or epithelial cells resulted in near complete suppression of pro-inflammatory cytokines, suggesting that IL-37 may act as a natural suppressor of the innate inflammatory and immune responses (2,3).
REFERENCES: 1) Boraschi D, Lucchesi D, Hainzl S, et al. IL-37: a new anti-inflammatory cytokine of the IL-1 family. Eur. Cytokine Netw. 2011; 22:127-47.
2) Nold MF, Nold-Petry CA, Zepp JA, et al. Interleukin 37 is a fundamental inhibitor of innate immunity. Nat. Immunol. 2010; 11:1014-22.
3) Sakai N, Van Sweringen HL, Belizaire RM, et al. Interleukin-37 reduces liver inflammatory injury via effects on hepatocytes and non-parenchymal cells. J. Gastroenterol. Hepatol. 2012; 27:1609-16.

For Research Use Only