Datasheet

IL-36G Antibody
CATALOG NUMBER: 7509

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:IL-36G antibody can be used for detection of IL-36G by Western blot at 1 - 2 μg/ml.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1306 - Human Spleen Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 19 kDa

Observed: 22 kDa
SPECIFICITY:IL-36G antibody is human specific. IL-36G antibody will not cross-react with IL-36A or IL-36B.
IMMUNOGEN:IL-36G antibody was raised against a 13 amino acid peptide near the carboxy terminus of human IL-36G.

The immunogen is located within the last 50 amino acids of IL-36G.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IL-36G antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IL-36G antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-36G antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IL-36G Antibody: IL1E, IL1F9, IL1H1, IL-1F9, IL-1H1, IL1RP2, IL-1RP2, IL1E, UNQ2456/PRO5737, Interleukin-36 gamma, IL-1-related protein 2
ACCESSION NO.:NP_062564
PROTEIN GI NO.:9665234
OFFICIAL SYMBOL:IL36G
GENE ID:56300

Background

BACKGROUND:IL-36G is is a member of the interleukin 1 cytokine family whose gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (1). IL-36G is thought to activate the NF-kappaB pathway through IL-1 receptor family members IL-1RL2 and IL-1RAcP (2). Like the related proteins IL-36A and IL-36B, IL-36G requires post-translational processing for full agonist activity, but the cleavage mechanism is currently unknown (3). IL-36G expression is stimulated by interferon-gamma, tumor necrosis factor-alpha and interleukin 1 (4). The IL-36 cytokines have been suggested to amplify Th1 responses by enhancing proliferation and Th1 polarization of naive CD4+ T cells (5).
REFERENCES: 1) Smith DE, Renshaw BR, Ketchem RR, et al. Four new members expand the interleukin-1 superfamily. J. Biol. Chem. 2000; 275:1169-75.
2) Towne JE, Garka KE, Renshaw BR, et al. Interleukin (IL)-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP to activate the pathway leading to NF-kappaB and MAPKs. J. Biol. Chem. 2004; 279:13677-88.
3) Towne JE, Renshaw BR, Douangpanya J, et al. Interleukin-36 (IL-36) ligands require processing for full agonist agonist (IL-36a, IL-36b, and IL-36g) or antagonist (IL-36Ra) activity. J. Biol. Chem. 2011; 286:42594-602.
4) Chustz RT, Nagarkar DR, Popowski JA, et al. Regulation and function of the IL-1 family cytokine IL-1F9 in human bronchial epithelial cells. Am. J. Respir. Cell Mol. Biol. 2011; 45:145-53.

For Research Use Only