Datasheet

IL-34 Antibody
CATALOG NUMBER: 4779

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (94%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:IL-34 antibody can be used for the detection of IL-34 by Western blot at 0.25 μg/mL. Despite its predicted molecular weight, IL-34 will often run at higher molecular weight in SDS-PAGE.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 26 kDa

Observed: 42-48 kDa
IMMUNOGEN:IL-34 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-34.

The immunogen is located within amino acids 30 - 80 of IL-34.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IL-34 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IL-34 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-34 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IL-34 Antibody: IL-34, C16orf77, Interleukin-34, IL-34
ACCESSION NO.:ACD13474
PROTEIN GI NO.:187610438
OFFICIAL SYMBOL:IL34
GENE ID:146433

Background

BACKGROUND:IL-34 Antibody: Interleukin-34 (IL-34) was identified through a functional screening of a library of secreted proteins after transfection of an embryonic kidney cell line with recombinant cDNAs. Flow cytometry and monocyte viability assays demonstrated increased survival and/or proliferation of monocytes induced by IL-34 and specific binding of IL-34 to CD14+ monocytes. IL-34 is a second ligand to the colony stimulating factor (CSF)-1 receptor in addition to CSF1. Like CSF1, IL-34 stimulates phosphorylation of ERK1 and ERK2 in human monocytes and induces differentiation of bone marrow cells into macrophages.
REFERENCES: 1) Lin H, Lee E, Hestir K, et al. Discovery of a cytokine and its receptor by functional screening of the extracellular proteome. Science2008; 320:807-11.

For Research Use Only