IL-33 Antibody Cat. No.: 4273

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (76.5%)
IMMUNOGEN: Anti-IL-33 antibody (4273) was raised against a 17 amino acid synthetic peptide from near the center of human IL-33.

The immunogen is located within the 150-200 amino acids of IL-33.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 1-2 μg/mL; IF: 20 μg/mL; IHC-P: 2.5 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1288 - A20 Cell Lysate
 2) Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 31kD

Observed: 33kD
psi-iconAdvanced Validation
VALIDATION:

Recombinant Protein Validation (Figure 2) shows expression of human partial recombinant protein IL-33 detected by anti-IL-33 antibody, 4273.  Bands of 18kD (monomer) and 36kD (dimer) were observed.

Regulated Expression Validation (Figure 7&8) shows the up-regulation (Figure 7) of IL-33 detected by anti-IL-33 antibody in human skin fibroblasts after α-irradiation and this effect  was inhibited by PPP (Figure 8).

ISOFORMS: Human IL-33 has 4 isoforms, including isoform 1 (270aa, 30.8kD), isoform 2 (228aa, 25.9kD), isoform 3 (228aa, 26.1kD) and isoform 4 (144aa, 16.3kD). This antibody detects only human isoform L. Mouse IL-33 has 1 isoform (266aa, 30kD). Rat IL-33 has only one isoform identified so far (264aa, 29.5kD).

psi-iconProperties
PURIFICATION:IL-33 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:IL-33 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-33 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:IL33
ALTERNATE NAMES:IL-33 Antibody: DVS27, IL1F11, NF-HEV, NFEHEV, C9orf26, NFHEV, Interleukin-33, Interleukin-1 family member 11, IL-33
ACCESSION NO.:NP_254274
PROTEIN GI NO.:15559209
GENE ID:90865
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:IL-33 Antibody: Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1α/beta, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-κB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties.
REFERENCES:1) Schmitz J et al. Immunity 2005; 23:479-90.
2) Dinarello Eur. Cytokine Netw. 2000; 11:483-6.
3) Brint et al. Nat. Immunol. 2004; 5:373-9.
4) Chackerian et al. J. Immunol. 2007; 179:2551-5.
psi-iconCITATIONS
CITATIONS: 1)Ivanov et al. Radiation-induced bystander signaling pathways in human fibroblasts: a role for interleukin-33 in the signal transmission.Cell Signal. 2010 Jul;22(7):1076-87. PMID: 20206688
2)Tsuda et al. Novel splice variants of IL-33: differential expression in normal and transformed cells. J Invest Dermatol. 2012 Nov;132(11):2661-4. PMID: 22785132

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