IL-32 Antibody Cat. No.: 3749

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: Anti-IL-32 antibody (3749) was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human IL-32.

The immunogen is located within the last 50 amino acids of IL-32.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 0.5-4 μg/mL; IHC: 2-10 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples; Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: This antibody detects all isoforms of IL-32.
POSITIVE CONTROL:1) Cat. No. 1306 - Human Spleen Tissue Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 26kD

Observed: 26 kD
psi-iconAdvanced Validation
VALIDATION:

Recombinant Protein Test (Figure 1): Anti-IL-32 antibodies (3749) detected human IL-32 recombinant protein at different concentrations.

ISOFORMS: Human IL-32 has 7 isoforms, including isoform 1 (234aa, 27kD), isoform 2 (188aa, 22kD), isoform 3 (178aa, 21kD), isoform 4 (131aa, 15kD), isoform 5 (168aa, 19kD), isoform 6 (179aa, 21kD) and isoform 7 (148aa, 17kD). 3749 can detect all human isoforms.

psi-iconProperties
PURIFICATION:IL-32 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:IL-32 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-32 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:IL32
ALTERNATE NAMES:IL-32 Antibody: NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd, IL-32beta, IL-32alpha, IL-32delta, IL-32gamma, NK4, Interleukin-32, Natural killer cells protein 4, IL-32
ACCESSION NO.:AAH09401
PROTEIN GI NO.:14424787
GENE ID:9235
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:IL-32 Antibody: Interleukin-32 (IL-32) was initially identified as a transcript (NK4) that is selectively expressed in lymphocytes and NK cells and whose expression is increased following activation by IL-2. It was later re-isolated from an IL-18-treated lung carcinoma cell line and re-named IL-32. IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-α, IL-8 and MIP-2, and because it is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFNγ, it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.
REFERENCES:1) Dahl et al. J. Immunol. 1992; 148:597-603.
2) Kim et al. Immunity 2005; 22:131-42.
3) Cagnard et al. Eur. Cyto. Network 2005; 16:289-92.

ANTIBODIES FOR RESEARCH USE ONLY.

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