IL-27 Antibody Cat. No.: 3799

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Pig: (92%), Rat: (79%)
IMMUNOGEN: Anti-IL-27 antibody (3799) was raised against a peptide corresponding to 14 amino acids near the center of human IL-27.

The immunogen is located within amino acids 80 - 130 of IL-27.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: WB: 2-4 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1288 - A20 Cell Lysate
 2) Cat. No. 17-207 - EL4 Cell Slide
 3) Cat. No. 1201 - HeLa Cell Lysate
 4) Cat. No. 1205 - Jurkat Cell Lysate
 5) Cat. No. 1220 - SK-N-SH Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 28 kDa

Observed: 28 kDa
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar IL-27 expression profile in human and mouse cell lines detected by two independent anti-IL-27 antibodies that recognize different epitopes, 3797 against N-terminus domain and 3799 against central domain.  IL-27 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Recombinant Protein Test (Figure 3) shows that anti-IL-27 antibody (3799) detects human recombinant IL-17 (IL-17A + IL-17B) protein that contains the amino acids of 3799 antigenic peptide.

ISOFORMS: Human IL-27 has one isoform (243aa, 28kD). Mouse IL-27 has one isoform (234aa, 27kD) and rat IL-27 also has one isoform (234aa, 27kD). 3797 can detect human, mouse and rat IL-27.

psi-iconProperties
PURIFICATION:IL-27 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:IL-27 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-27 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:IL27
ALTERNATE NAMES:IL-27 Antibody: p28, IL30, IL-27, IL27A, IL-27A, IL27p28, Interleukin-27 subunit alpha, p28, IL-27 subunit alpha
ACCESSION NO.:NP_663634
PROTEIN GI NO.:28416913
GENE ID:246778
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:IL-27 Antibody: Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet.
REFERENCES:1) Pfanz et al. Immunity 2002; 16:779-90.
2) Pfanz et al. J. Immunol. 2004; 172:2225-31.
3) Hisada et al. Cancer Res. 2004; 64:1152-6.

ANTIBODIES FOR RESEARCH USE ONLY.

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