| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Horse: (94%), Pig: (82%), Rat: (76%), Guinea pig: (76%) |
| IMMUNOGEN: | Anti-IL-23 antibody (3795) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human IL-23. The immunogen is located within amino acids 40 - 90 of IL-23. |
| TESTED APPLICATIONS: | ELISA, IF, IHC-P, WB |
| APPLICATIONS: | WB: 0.25-2 μg/mL; IF: 20 μg/mL, IHC: 2 μg/mL. Antibody validated: Western Blot in human and mouse samples; Immunofluorescence and Immunohistochemistry in mouse samples. All other applications and species not yet tested. |
| POSITIVE CONTROL: | 1) Cat. No. 1414 - Mouse Pancreas Tissue Lysate |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 21kD Observed: 22 kD |
Specifications| VALIDATION: | Recombinant Protein Test (Figure 2): Anti-IL-23 antibodies (3795) detected human IL-23 recombinant protein at different concentrations.
Induced Expression Validation (Figure 6): IL-23 expression detected by anit-AIF antibodies was up-regulated by tape stripping. |
| ISOFORMS: | Human IL-23 has one isoform (189aa, 20.7kD). Mouse IL-23 has one isoform (196aa, 22.1kD) and Rat IL-23 also has one isoform (196aa, 22kD). 3795 can detect human and mouse, and possibly detect rat. |
| PURIFICATION: | IL-23 Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | IL-23 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | IL-23 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | IL23A |
| ALTERNATE NAMES: | IL-23 Antibody: P19, SGRF, IL-23, IL-23A, IL23P19, UNQ2498/PRO5798, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, IL-23 subunit alpha |
| ACCESSION NO.: | AAH66268 |
| PROTEIN GI NO.: | 42542809 |
| GENE ID: | 51561 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | IL-23 Antibody: Like interleukin-27 (IL-27), IL-23 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the IL-12p40 subunit and a novel p19 subunit. IL-23 is secreted by activated dendritic cells, macrophages, and monocytes. Its biological activities include enhancing the proliferation of memory T cells and the production of IFN-gamma, IL-12, and TNF-α from activated T cells, and can stimulate macrophages to produce TNF-α and nitric oxide. It has also been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer. |
| REFERENCES: | 1) Hunter. Nat. Rev. Immunol. 2005; 5:521-31. | 2) Oppmann et al. Immunity 2000; 13:715-25. | 3) Sheibanie et al. FASEB J. 2004; 18:1318-20. | 4) Pirhonen et al. J. Immunol. 2002; 169:5673-8. |
| CITATIONS: | 1)Yoon et al. IL-23 induced in keratinocytes by endogenous TLR4 ligands polarizes dendritic cells to drive IL-22 responses to skin immunization. J Exp Med. 2016;213(10):2147-66. PMID: 27551155 |
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