IL-23 Antibody Cat. No.: 3795

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Horse: (94%), Pig: (82%), Rat: (76%), Guinea pig: (76%)
IMMUNOGEN: IL-23 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-23.

The immunogen is located within amino acids 40 - 90 of IL-23.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: IL-23 antibody can be used for the detection of IL-23 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1414 - Mouse Pancreas Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 21 kDa

Observed: 23 kDa

psi-iconProperties
PURIFICATION:IL-23 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:IL-23 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-23 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:IL23A
ALTERNATE NAMES:IL-23 Antibody: P19, SGRF, IL-23, IL-23A, IL23P19, UNQ2498/PRO5798, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, IL-23 subunit alpha
ACCESSION NO.:AAH66268
PROTEIN GI NO.:42542809
GENE ID:51561
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:IL-23 Antibody: Like interleukin-27 (IL-27), IL-23 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the IL-12p40 subunit and a novel p19 subunit. IL-23 is secreted by activated dendritic cells, macrophages, and monocytes. Its biological activities include enhancing the proliferation of memory T cells and the production of IFN-gamma, IL-12, and TNF-α from activated T cells, and can stimulate macrophages to produce TNF-α and nitric oxide. It has also been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.
REFERENCES:1) Hunter CA. New IL-12-family members: IL-23 and IL-27, cytokines with divergent functions. Nat. Rev. Immunol. 2005; 5:521-31.
2) Oppmann B, Lesley R, Blom B, et al. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12. Immunity 2000; 13:715-25.
3) Sheibanie AF, Tadmori I, Jing H, et al. Prostaglandin ED induces IL-23 production in bone marrow-derived dendritic cells. FASEB J. 2004; 18:1318-20.
4) Pirhonen J, Matikainen S, Julkunen I. Regulation of virus-induced IL-12 and IL-23 expression in human macrophages. J. Immunol. 2002; 169:5673-8.

ANTIBODIES FOR RESEARCH USE ONLY.

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