| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Horse: (94%), Pig: (82%), Rat: (76%), Guinea pig: (76%) |
| IMMUNOGEN: | IL-23 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-23. The immunogen is located within amino acids 40 - 90 of IL-23. |
| TESTED APPLICATIONS: | ELISA, IF, IHC-P, WB |
| APPLICATIONS: | IL-23 antibody can be used for the detection of IL-23 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
| POSITIVE CONTROL: | 1) Cat. No. 1414 - Mouse Pancreas Tissue Lysate |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 21 kDa Observed: 23 kDa |
Specifications| PURIFICATION: | IL-23 Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | IL-23 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | IL-23 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | IL23A |
| ALTERNATE NAMES: | IL-23 Antibody: P19, SGRF, IL-23, IL-23A, IL23P19, UNQ2498/PRO5798, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, IL-23 subunit alpha |
| ACCESSION NO.: | AAH66268 |
| PROTEIN GI NO.: | 42542809 |
| GENE ID: | 51561 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | IL-23 Antibody: Like interleukin-27 (IL-27), IL-23 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the IL-12p40 subunit and a novel p19 subunit. IL-23 is secreted by activated dendritic cells, macrophages, and monocytes. Its biological activities include enhancing the proliferation of memory T cells and the production of IFN-gamma, IL-12, and TNF-α from activated T cells, and can stimulate macrophages to produce TNF-α and nitric oxide. It has also been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer. |
| REFERENCES: | 1) Hunter CA. New IL-12-family members: IL-23 and IL-27, cytokines with divergent functions. Nat. Rev. Immunol. 2005; 5:521-31. | 2) Oppmann B, Lesley R, Blom B, et al. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12. Immunity 2000; 13:715-25. | 3) Sheibanie AF, Tadmori I, Jing H, et al. Prostaglandin ED induces IL-23 production in bone marrow-derived dendritic cells. FASEB J. 2004; 18:1318-20. | 4) Pirhonen J, Matikainen S, Julkunen I. Regulation of virus-induced IL-12 and IL-23 expression in human macrophages. J. Immunol. 2002; 169:5673-8. |
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