IL-23 Antibody Cat. No.: 3793

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Guinea pig: (93%), Horse: (93%), Mouse: (71%), Rat: (71%)
IMMUNOGEN: Anti-IL-23 antibody (3793) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of mature human IL-23.

The immunogen is located the last 50 amino acids of IL-23.
TESTED APPLICATIONS: ELISA, ICC, IF, IHC-P, WB
APPLICATIONS: WB: 1-4 μg/mL; ICC: 10 μg/mL; IF: 20 μg/mL, IHC: 5 μg/mL.

Antibody validated: Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1207 - Raji Cell Lysate
 2) Cat. No. 11-101 - Human Pancreas Tissue Slide
 3) Cat. No. 17-007 - Raji Cell Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 21kD

Observed: 22 kD
psi-iconAdvanced Validation
VALIDATION:

Recombinant Protein Test (Figure 3): Anit-IL-23 antibodies (3793) detected human IL-23 recombinant protein.

 

Regulated expression validation (Figure 8): IL-23 expression detected by anit-IL-23 antibodies (3793) was abundant in human abdominal aortic aneurysm (AAA) tissues whereas there is no IL-23 expression in normal tissue.

ISOFORMS: Human IL-23 has one isoform (189aa, 20.7kD). Mouse IL-23 has one isoform (196aa, 22.1kD) and Rat IL-23 also has one isoform (196aa, 22kD). 3793 can detect human IL-23.

psi-iconProperties
PURIFICATION:IL-23 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:IL-23 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-23 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:IL23A
ALTERNATE NAMES:IL-23 Antibody: P19, SGRF, IL-23, IL-23A, IL23P19, UNQ2498/PRO5798, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, IL-23 subunit alpha
ACCESSION NO.:AAH66268
PROTEIN GI NO.:42542809
GENE ID:51561
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:IL-23 Antibody: Like interleukin-27 (IL-27), IL-23 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the IL-12p40 subunit and a novel p19 subunit. IL-23 is secreted by activated dendritic cells, macrophages, and monocytes. Its biological activities include enhancing the proliferation of memory T cells and the production of IFN-gamma, IL-12, and TNF-α from activated T cells, and can stimulate macrophages to produce TNF-α and nitric oxide. It has also been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.
REFERENCES:1) Hunter. Nat. Rev. Immunol. 2005; 5:521-31.
2) Oppmann et al. Immunity 2000; 13:715-25.
3) Sheibanie et al. FASEB J. 2004; 18:1318-20.
4) Pirhonen et al. J. Immunol. 2002; 169:5673-8.
psi-iconCITATIONS
CITATIONS: 1)Yan et al. Interleukin-12 and -23 blockade mitigates elastase-induced abdominal aortic aneurysm. Sci Rep. 2019;9(1):10447.PMID: 31320700

ANTIBODIES FOR RESEARCH USE ONLY.

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