Datasheet

IL-22 Receptor Antibody
CATALOG NUMBER: 2497

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (93%), Bovine: (88%)
TESTED APPLICATIONS:ELISA, ICC, IF, IHC-P, WB
APPLICATIONS:IL-22 receptor antibody can be used for detection of IL-22 receptor by Western blot at 0.5-1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.               

Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples; Immunocytochemistry in human samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1211 - HepG2 Cell Lysate
 2) Cat. No. 17-011 - Hep G2 Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 63 kDa

Observed: 65 kDa
IMMUNOGEN:IL-22 receptor antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human IL-22 receptor precursor.

The immunogen is located within the first 50 amino acids of IL-22 Receptor.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IL-22 Receptor Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IL-22 Receptor Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-22 Receptor antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IL-22 Receptor Antibody: IL22R, CRF2-9, IL22R1, IL22R, Interleukin-22 receptor subunit alpha-1, Cytokine receptor class-II member 9, IL-22 receptor subunit alpha-1
ACCESSION NO.:NP_067081
PROTEIN GI NO.:10719607
OFFICIAL SYMBOL:IL22RA1
GENE ID:58985

Background

BACKGROUND:IL-22 Receptor Antibody: A novel cytokine, designated IL-TIF for IL-10 related T cell-derived inducible factor and IL-22, was recently identified. The receptor for IL-22 (IL-22R, also termed CRF2-9 and IL-TIF-R1 chain) is a new member of the class II cytokine receptor family. IL-22R forms a complex with IL-10 receptor beta chain and mediates IL-22 signaling. IL-22 and its receptor activate JAK-STAT signaling pathway. IL22R is expressed in normal liver and kidney and their cell lines HepG2 and TK-10. A soluble form of IL-22 receptor, also termed IL-22 binding protein (IL-22BP) and IL-22 receptor-alpha 2 (IL-22RA2), was identified very recently. IL-22BP prevents binding of IL-22 to the functional cell surface IL-22R complex and neutralizes IL-22 activity. LPS induces IL-22 expression, which indicates the role of IL-22 in inflammatory response.
REFERENCES: 1) Dumoutier L, Louahed J, Renauld JC. Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9. J Immunol. 2000;164(4):1814-9.
2) Dumoutier L, Van Roost E, Colau D, Renauld JC.  Human interleukin-10-related T cell-derived inducible factor: molecular cloning and functional characterization as an hepatocyte-stimulating factor. Proc Natl Acad Sci USA. 2000 ;97(18):10144-9.
3) Xie MH, Aggarwal S, Ho WH, et al. Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R. J Biol Chem. 2000;275(40):31335-9.
4) Kotenko SV, Izotova LS, Mirochnitchenko OV, et al. Identification of the functional interleukin-22 (IL-22) receptor complex: J Biol Chem. 2001;276(4):2725-32.

For Research Use Only