Datasheet

IL-22 Antibody
CATALOG NUMBER: 7593

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:IL-22 antibody can be used for detection of IL-22 by Western blot at 0.5 μg/ml. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 20 kDa

Observed: 17 kDa
SPECIFICITY:IL-22 antibody is human, mouse, and rat reactive. Multiple isoforms of IL-22 are known to exist.
IMMUNOGEN:IL-22 antibody was raised against a 17 amino acid peptide near the center of human IL-22 .

The immunogen is located within amino acids 90 - 140 of IL-22.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IL-22 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IL-22 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-22 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IL-22 Antibody: TIFa, IL-21, IL-22, ILTIF, IL-TIF, IL-D110, zcyto18, TIFIL-23, ZCYTO18, UNQ3099/PRO10096, Interleukin-22, Cytokine Zcyto18
ACCESSION NO.:NP_065386
PROTEIN GI NO.:10092625
OFFICIAL SYMBOL:IL22
GENE ID:50616

Background

BACKGROUND:Interleukin-22 (IL-22) is a cytokine important for the modulation of tissue responses during inflammation (1). Unlike the distantly related IL-10, IL-22 does not inhibit the production of proinflammatory cytokines in monocytes in response to LPS, but it has some inhibitory effects on IL-4 production from Th2 T cells. IL-22 is expressed by both the adaptive arm of the immune system such as CD4 T cell subsets including Th17 cells, as well as by innate lymphocytes such as NK and LTi-like cells (2). IL-22 is highly expressed in several chronic inflammatory conditions, and studies suggest that IL-22 plays both inflammatory and protective roles (3).
REFERENCES: 1) Xie MH, Aggarwal S, Ho WH, et al. Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R. J. Biol. Chem. 2000; 275:31335-9.
2) Wolk K, Kunz S, Witte E, et al. IL-22 increases the innate immunity of tissues. Immunity 2004; 21:241-54.
3) Wolk K, Witte E, Wallace E, et al. IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis. Eur. J. Immunol. 36:1309-23.

For Research Use Only