Datasheet

IL-21 Receptor Antibody
CATALOG NUMBER: 2469

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:IL-21 receptor antibody can be used for detection of IL-21 receptor by Western blot at 1-2 μg/mL. An approximately 60 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1207 - Raji Cell Lysate
 2) Cat. No. 1462 - Rat Lung Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 60 kDa

Observed: 62 kDa        
IMMUNOGEN:IL-21 receptor antibody was raised against a synthetic peptide corresponding to amino acids 97 to 111 of human IL-21 receptor precursor.

The immunogen is located within amino acids 80 - 130 of IL-21 Receptor.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IL-21 Receptor Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IL-21 Receptor Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:IL-21 Receptor antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IL-21 Receptor Antibody: NILR, CD360, NILR, UNQ3121/PRO10273, Interleukin-21 receptor, Novel interleukin receptor, IL-21 receptor
ACCESSION NO.:NP_068570
PROTEIN GI NO.:11141869
OFFICIAL SYMBOL:IL21R
GENE ID:50615

Background

BACKGROUND:IL-21 Receptor Antibody: A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gammac, and mediates IL-21 signaling. Both IL-21R and the gammac are necessary for the IL-21 function. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
REFERENCES: 1) Parrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature. 2000;408(6808):57-63.
2) Ozaki K, Kikly K, Michalovich D, et al.  Cloning of a type I cytokine receptor most related to the IL-2 receptor β chain. Proc Natl Acad Sci USA. 2000;97(21):11439-44.
3) Asao H, Okuyama C, Kumaki S, et al. the common γ-chain is an indispensable subunit of the IL-21 receptor complex. J Immunol. 2001;167(1):1-5.
4) Vosshenrich CA, Di Santo JP. Cytokines: IL-21 joins the γ(c)-dependent network.Curr Biol. 2001;11(5):R175-7.

For Research Use Only