| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| IMMUNOGEN: | IL-21 receptor antibody was raised against a synthetic peptide corresponding to amino acids 97 to 111 of human IL-21 receptor precursor. The immunogen is located within amino acids 80 - 130 of IL-21 Receptor. |
| TESTED APPLICATIONS: | ELISA, IF, IHC-P, WB |
| APPLICATIONS: | IL-21 receptor antibody can be used for detection of IL-21 receptor by Western blot at 1-2 μg/mL. An approximately 60 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested. |
| POSITIVE CONTROL: | 1) Cat. No. 1207 - Raji Cell Lysate |
| 2) Cat. No. 1462 - Rat Lung Tissue Lysate | |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 60 kDa Observed: 62 kDa |
Specifications| PURIFICATION: | IL-21 Receptor Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | IL-21 Receptor Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | batch dependent |
| STORAGE CONDITIONS: | IL-21 Receptor antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | IL21R |
| ALTERNATE NAMES: | IL-21 Receptor Antibody: NILR, CD360, NILR, UNQ3121/PRO10273, Interleukin-21 receptor, Novel interleukin receptor, IL-21 receptor |
| ACCESSION NO.: | NP_068570 |
| PROTEIN GI NO.: | 11141869 |
| GENE ID: | 50615 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | IL-21 Receptor Antibody: A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gammac, and mediates IL-21 signaling. Both IL-21R and the gammac are necessary for the IL-21 function. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations. |
| REFERENCES: | 1) Parrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature. 2000;408(6808):57-63. | 2) Ozaki K, Kikly K, Michalovich D, et al. Cloning of a type I cytokine receptor most related to the IL-2 receptor β chain. Proc Natl Acad Sci USA. 2000;97(21):11439-44. | 3) Asao H, Okuyama C, Kumaki S, et al. the common γ-chain is an indispensable subunit of the IL-21 receptor complex. J Immunol. 2001;167(1):1-5. | 4) Vosshenrich CA, Di Santo JP. Cytokines: IL-21 joins the γ(c)-dependent network.Curr Biol. 2001;11(5):R175-7. |
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