The extracellular domain of mouse IL-2 (aa 21-169) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a.
FUSION TAG:
Fc Tag
TESTED APPLICATIONS:
APPLICATIONS:
This recombinant proteins is for research use only.
BIOLOGICAL ACTIVITY:
Specific Activity: 1-1.5 x106 Units/mg, measured using CTLL indicator cells.
Shows the biological function of the IL-2 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain. Blocks diabetogenic autoimmunity in nonobese diabetic mice.
Properties
PURITY:
>98% (SDS-PAGE).
Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza).
PHYSICAL STATE:
Lyophilized
BUFFER:
Lyophilized from 0.2um-filtered solution in PBS. Reconstitute at 100 μg/ml in sterile PBS.
STORAGE CONDITIONS:
Stable for at least 1 year after receipt when stored at -20˚C. Working aliquots are stable for up to 3 months when stored at -20˚C.
Additional Info
ALTERNATE NAMES:
Interleukin-2, Aldesleukin
ACCESSION NO.:
NP_032392
PROTEIN GI NO.:
7110653
OFFICIAL SYMBOL:
Il2
GENE ID:
16183
Background and References
BACKGROUND:
Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells, and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils, and induces mononuclear cells to secrete IFN-gamma and TNF-alpha and -beta. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important hemeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.
Specifications
