| HOST SPECIES: | Rat |
| SPECIES REACTIVITY: | Mouse |
| IMMUNOGEN: | E.coli derived Recombinant Murine IL-2 |
| TESTED APPLICATIONS: | ELISA, Neut, WB |
| APPLICATIONS: | ELISA: Sandwich: In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with ProSci’s Polyclonal biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 μg/mL. Western Blot: To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL), a concentration of 10.0-14.0 μg/mL of this antibody is required. |
Specifications| PURIFICATION: | This IgG2Ak antibody was purified from cell culture by Protein A affinity chromatography. |
| CLONALITY: | Monoclonal |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Lyophilized |
| STORAGE CONDITIONS: | The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| OFFICIAL SYMBOL: | IL2 |
| ALTERNATE NAMES: | Interleukin 2Interleukin-2, T-cell growth factor, IL-2, TCGF, lymphokine |
| ACCESSION NO.: | P60568 |
| PROTEIN GI NO.: | 45593462 |
| GENE ID: | 3558 |
| USER NOTE: | Centrifuge vial prior to opening. |
ANTIBODIES FOR RESEARCH USE ONLY.
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