IL-2 Antibody [5B9-2-1] Cat. No.: 38-299

Availability: In stock

$0.00

 Price Options

* Required Fields

$0.00
psi-iconSpecifications
HOST SPECIES:Rat
SPECIES REACTIVITY: Mouse
IMMUNOGEN: E.coli derived Recombinant Murine IL-2
TESTED APPLICATIONS: ELISA, Neut, WB
APPLICATIONS: ELISA:
Sandwich:
In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with ProSci’s Polyclonal biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 μg/mL.

Western Blot:

To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.

Neutralization:

To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL), a concentration of 10.0-14.0 μg/mL of this antibody is required.

psi-iconProperties
PURIFICATION:This IgG2Ak antibody was purified from cell culture by Protein A affinity chromatography.
CLONALITY:Monoclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Lyophilized
STORAGE CONDITIONS:The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:IL2
ALTERNATE NAMES:Interleukin 2Interleukin-2, T-cell growth factor, IL-2, TCGF, lymphokine
ACCESSION NO.:P60568
PROTEIN GI NO.:45593462
GENE ID:3558
USER NOTE:Centrifuge vial prior to opening.

ANTIBODIES FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.