HOST SPECIES: | Mouse |
SPECIES REACTIVITY: | Rat |
IMMUNOGEN: | E.coli derived Recombinant Rat IL-2 |
TESTED APPLICATIONS: | ELISA, WB |
APPLICATIONS: | ELISA: Sandwich: In a sandwich ELISA (assuming 100μL/well), a concentration of 14.0-16.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant rat IL-2 when used with ProSci’s Polyclonal biotinylated antigen affinity purified anti-rat IL-2 (38-205) as the detection antibody at a concentration of at least 2.0-4.0 μg/mL. Western Blot: To detect rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions. |
PURIFICATION: | This IgG1k antibody was purified from cell culture by Protein A affinity chromatography. |
CLONALITY: | Monoclonal |
CONJUGATE: | Unconjugated |
PHYSICAL STATE: | Lyophilized |
STORAGE CONDITIONS: | The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
OFFICIAL SYMBOL: | IL2 |
ALTERNATE NAMES: | Interleukin 2Interleukin-2, T-cell growth factor, IL-2, TCGF, lymphokine |
ACCESSION NO.: | P60568 |
PROTEIN GI NO.: | 45593462 |
GENE ID: | 3558 |
USER NOTE: | Centrifuge vial prior to opening. |
ANTIBODIES FOR RESEARCH USE ONLY.
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