IL-1F10 Antibody Cat. No.: 7503

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse (100%) and Rat (88%).
IMMUNOGEN: Anti-IL-1F10 antibody (7503) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human IL-1F10.

The immunogen is located within the last 50 amino acids of IL-1F10.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: WB: 1 μg/mL; IHC: 5 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples. All other applications and species not yet tested.
SPECIFICITY: IL-1F10 antibody is human, mouse and rat reactive. Two isoforms of IL-1F10 exist.
POSITIVE CONTROL:1) Cat. No. 1304 - Human Liver Tissue Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 17kD

Observed: 17 kD
psi-iconAdvanced Validation
VALIDATION:

Recombinant Protein Test (Figure 1): Anti- IL-1F10  antibodies (7503) detected human IL-1F10  recombinant protein at different concentrations.

ISOFORMS: Human IL-1F10 has 2 isoforms, including isoform 1 (152aa, 17kD) and isoform 2 (152aa, 17kD). Mouse IL-1F10 has one isoform (152aa, 17kD) and Rat IL-1F10 also has one isoform (152aa, 17kD). 7503 can detect human, mouse and rat.

psi-iconProperties
PURIFICATION:IL-1F10 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:IL-1F10 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-1F10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:IL1F10
ALTERNATE NAMES:IL-1F10 Antibody: IL-38, FKSG75, IL1HY2, IL-1HY2, IL1-theta, FIL1-theta, FIL1T, IL38, UNQ6119/PRO20041, Interleukin-1 family member 10, Family of interleukin 1-theta, IL-1F10
ACCESSION NO.:NP_775184
PROTEIN GI NO.:27894307
GENE ID:84639
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The cytokine IL-1 is responsible for initiating a variety of activities through the activation of transcription factors, NF-kappa B and AP-1, thereby promoting host response to injury or infection (1). IL-1F10 (Interleukin-1 family member 10), also known as FIL1T, IL1HY2 or FKSG75, is highly expressed in the skin, spleen and proliferating B-cells of the tonsil (2). IL-1F10 binds soluble IL-1 receptor type 1, and is one of nine Interleukin 1 families clustered on chromosome 2, where it is thought to participate in the regulation of adapted and innate immune responses (3). IL-1F10 and IL-1RN polymorphisms may play an important role in the susceptibility to developing rheumatoid arthritis (4).
REFERENCES:1) Towne et al. J. Biol. Chem. 2004; 279:13677-88.
2) Lin et al. J. Biol. Chem. 2001; 276: 20597-602.
3) Bensen et al. J. Interferon Cytokine Res. 2001; 21:899-904.
4) Cruz-Robles et al. Immunol. Invest. 2009; 38:231-9.

ANTIBODIES FOR RESEARCH USE ONLY.

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