IL-17 Antibody Cat. No.: 4887

Availability: In stock

$0.00

 Price Options

* Required Fields

$0.00
psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (84%), Rat: (81%), Pig: (74%)
IMMUNOGEN: Anti-IL-17 antibody (4887) was raised against a peptide corresponding to 16 amino acids near the center of mature human IL-17.

The immunogen is located within amino acids 50-100 of IL-17.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 0.125-0.5 μg/mL; IF: 5-20 μg/mL; IHC: 2-5 μg/mL.

Antibody validated: Western Blot in human samples; Immunofluorescence in mouse samples; Immunohistochemistry in human and mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 17-208 - A20 Cell Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 17.5kD

Observed: 20 kD (Post-modification: 1 N-linked glycosylation)
psi-iconAdvanced Validation
VALIDATION:

Recombinant Protein Test (Figure 1): Anit-IL-17 antibodies (4887) detected human IL-17 recombinant protein at different concentrations

ISOFORMS: Human IL-17 has only one isoform (155aa, 17.5kD). Mouse IL-17 has one isoform (158aa, 17.5kD) and Rat IL-17 also has one isoform (150aa, 16.9kD). 4887 can detect human, mouse and rat IL-17.

psi-iconProperties
PURIFICATION:IL-17 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:IL-17 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-17 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:IL17A
ALTERNATE NAMES:IL-17 Antibody: IL17, CTLA8, IL-17, IL-17A, IL17, Interleukin-17A, Cytotoxic T-lymphocyte-associated antigen 8
ACCESSION NO.:NP_002181
PROTEIN GI NO.:4504651
GENE ID:3605
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:IL-17 Antibody: Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-κB and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.
REFERENCES:1) Miossec. Curr. Opin. Rheumatol.2000; 12:181-5.
2) Paunovic et al. Rheumatol.2008; 47:771-6.
3) Steinman. Nat. Med.2007; 13:139-145.

ANTIBODIES FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.