Datasheet

IL-17 Antibody
CATALOG NUMBER: 4877

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:IL-17 antibody can be used for the detection of IL-17 by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1208 - THP-1 Cell Lysate
 2) Cat. No. 1288 - A20 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 17 kDa

Observed: 17 kDa
IMMUNOGEN:IL-17 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-17A.

The immunogen is located within amino acids 40 - 90 of IL-17.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IL-17 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IL-17 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-17 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IL-17 Antibody: IL17, CTLA8, IL-17, IL-17A, IL17, Interleukin-17A, Cytotoxic T-lymphocyte-associated antigen 8
ACCESSION NO.:NP_002181
PROTEIN GI NO.:4504651
OFFICIAL SYMBOL:IL17A
GENE ID:3605

Background

BACKGROUND:IL-17 Antibody: Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-κB and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.
REFERENCES: 1) Miossec P. Are T cells in rheumatoid synovium aggressors or bystanders? Curr. Opin. Rheumatol.2000; 12:181-5.
2) Paunovic V, Carroll HP, Vandenbroeck K, et al. Crossed signals: the role of interleukin (IL)-12, -17, -23, and -27 in autoimmunity. Rheumatol.2008; 47:771-6.
3) Steinman L. A brief history of TH17, the first major revision in the TH1/TH2 hypothesis of T cell-mediated tissue damage. Nat. Med.2007; 13:139-145.

For Research Use Only