Datasheet

IL-16 Antibody
CATALOG NUMBER: 4995

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:IL-16 antibody can be used for detection of IL-16 by Western blot at 2.5 - 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 145 kDa

Observed: 160 kDa
SPECIFICITY:This antibody will only detect the NIL-16 isoform.
IMMUNOGEN:IL-16 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human IL-16.

The immunogen is located within amino acids 140 - 190 of IL-16.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IL-16 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IL-16 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-16 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IL-16 Antibody: LCF, NIL16, PRIL16, prIL-16, Pro-interleukin-16, Lymphocyte chemoattractant factor, IL-16
ACCESSION NO.:AAQ86961
PROTEIN GI NO.:36953836
OFFICIAL SYMBOL:IL16
GENE ID:3603

Background

BACKGROUND:IL-16 Antibody: IL-16 was initially identified as a chemotactic cytokine, but is now known to possess a wide range of activities. Later studies have more fully characterized IL-16 as an immunomodulatory cytokine that contributes to the regulatory process of CD4+ T cell recruitment and activation at sites of inflammation in association with asthma and several autoimmune diseases. The precursor of IL-16 (pro-IL-16) is thought to be cleaved towards the C-terminal region by Caspase-3, releasing a 20 kDa active form that binds to and signals through CD4. Besides acting as a chemotactic cytokine, IL-16 is thought to also be involved in the regulation of T cell proliferation and multiple infectious, immune-mediated, and autoimmune inflammatory disorders including irritable bowel syndrome, systemic lupus erythematosus, and neurodegenerative disorders. At least two isoforms of IL-16 are known to exist; the longer isoform (also known as NIL-16) is detected only in neurons of the cerebellum and hippocampus.
REFERENCES: 1) Cruikshank WW, Center DM, Nisar N, et al. Molecular and functional analysis of a lymphocyte chemoattractant factor: association of biologic function with CD expression. Proc. Natl. Acad. Sci. USA1994; 91:5109-13.
2) Interleukin-16. Cruikshank WW, Kornfeld H, and Center DM. J. Leukoc. Biol.2000; 67:757-66.
3) Zhang Y, Center DM, Wu DM, et al. Processing and activation of pro-interleukin-16 by caspase-3. J. Biol. Chem.1998; 273:1144-9.
4) Maciaszek JW, Parada NA, Cruikshank WW, et al. IL-16 represses HIV-1 promoter activity. J. Immunol.1997; 158:5-8.

For Research Use Only