Datasheet

IL-15 Antibody
CATALOG NUMBER: 7231

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Guinea pig: (100%), Sheep: (92%), Rabbit: (92%), Pig: (92%), Bovine: (92%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:IL-15 antibody can be used for detection of IL-15 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in rat samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1466 - Rat Spleen Tissue Lysate
PREDICTED MOLECULAR WEIGHT:18 kDa
SPECIFICITY:At least two isoforms of IL-15 are known to exist.
IMMUNOGEN:Rabbit polyclonal IL-15 antibody was raised against a 13 amino acid peptide near the center of human IL-15.

The immunogen is located within amino acids 50 - 100 of IL-15.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IL-15 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IL-15 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-15 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IL-15 Antibody: IL-15, Interleukin-15, IL-15
ACCESSION NO.:NP_000576
PROTEIN GI NO.:10835153
OFFICIAL SYMBOL:IL15
GENE ID:3600

Background

BACKGROUND:IL-15 Antibody: Interleukin 15 (IL-15) is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL-2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis.
REFERENCES: 1) Bodnar A, Nizsaloczki E, Mocsar G, et al. A biophysical approach to IL-2 and IL-15 receptor function: localization, conformation and interactions. Immunol. Lett. 2008; 116:117-25.
2) Ku CC, Murakami M, Sakamoto A, et al. Control of homeostasis of CD8+ memory T cells by opposing cytokines. Science 2000; 288:675-8.
3) Waldmann T, Tagaya Y, and Bamford R. Interleukin-2, interleukin-15, and their receptors. Int. Rev. Immunol. 1998; 16:205-26.
4) Masuda A, Matsuguchi T, Yamaki K, et al. Interleukin-15 prevents mouse mast cell apoptosis through STAT6-mediate Bcl-xL expression. J. Biol. Chem. 2001; 276:26107-13.

For Research Use Only