Datasheet

IL-12A Antibody
CATALOG NUMBER: 7497

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Llama: (94%), Goat: (89%), Pig: (89%), Sheep: (89%), Horse: (83%), Bovine: (83%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:IL-12A antibody can be used for detection of IL-12A by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 22 kDa

Observed: 22 kDa
SPECIFICITY:IL-12A antibody is human specific. IL-12A antibody will not cross-react with IL-12B.
IMMUNOGEN:IL-12A antibody was raised against an 18 amino acid peptide near the carboxy terminus of human IL-12A.

The immunogen is located within the last 50 amino acids of IL-12A.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IL-12A antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IL-12A antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-12A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Interleukin 12 alpha, p35, natural killer cell stimulatory factor 1, NKSF1, cytotoxic lymphocyte maturation factor 1, CLMF, NF cell stimulatory factor chain 1, NFSK
ACCESSION NO.:NP_000873
PROTEIN GI NO.:24430219
OFFICIAL SYMBOL:IL 12A
GENE ID:3592

Background

BACKGROUND:IL-12A is subunit of the cytokine IL-12 which acts on T- and natural killer cells, and has a broad array of biological activities. IL-12 is a disulfide-linked heterodimer composed of the 35-kD subunit and a 40-kD subunit that is a member of the cytokine receptor family (1). IL-12 is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells (1,2). The responses of lymphocytes to IL-12 are mediated by the activator of transcription protein STAT4 (2). Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity (3).
REFERENCES: 1) Gee K, Guzzo C, Che Mat NF, et al. The IL-12 family of cytokines in infection, inflammation and autoimmune disorders. Inflamm. Allergy Drug Targets 2009; 8:40-52.
2) Sinigaglia F, D’Ambrosio D, Panina-Bordignon P, et al. Regulation of the IL-12/IL-12R axis: a critical step in T-helper cell differentiation and effector function. Immunol. Rev. 1999; 170:65-72.
3) Bogdan C, Rollinghoff M, and Diefenbach A. The role of nitric oxide in innate immunity. Immunol. Rev. 2000; 173:17-26.

For Research Use Only