Datasheet

IL-11 Antibody
CATALOG NUMBER: 8453

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (94%), Mouse: (88%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:IL-11 antibody can be used for the detection of IL-11 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 10 μg/ml.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 20 kDa

Observed: 19 kDa
SPECIFICITY:IL-11 antibody is human specific. At least two isoforms of IL-11 are known to exist; this antibody will only detect the longest isoform of IL-11.
IMMUNOGEN:IL-11 antibody was raised against a 16 amino acid peptide from near the amino terminus of human IL-11.

The immunogen is located within amino acids 40 - 90 of IL-11.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IL-11 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IL-11 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-11 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Interleukin-11, Adipogenesis inhibitory factor, AGIF
ACCESSION NO.:NP_000632
PROTEIN GI NO.:10834994
OFFICIAL SYMBOL:IL-11
GENE ID:3589

Background

BACKGROUND:Interleukin 11 is a pleiotropic cytokine that has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6 (1). It directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (2). IL-11 promotes the proliferation of hepatocytes in response to liver damage. IL-11 binds to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 (3). Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelo-suppressive chemotherapy (4).
REFERENCES: 1) Paul SR, Bennett F, Calvetti JA. Molecular cloning of a cDNA encoding interleukin-11, astromal cell-derived lymphopoietic and hematopoietic cytokine. Proc. Natl. Acad. Sci. USA 1990; 87:7512-6.
2) Yang YC and Yin T. Interleukin (IL)-11 mediated signal transduction. Ann. N.Y. Acad. Sci. 1995; 762:31-40.
3) Dimitriadis E, Robb L, Salamonsen LA. Interleukin 11 advances progesterone-induced decidualization of human endometrial stromal cells. Mol. Hum. Reprod. 2002; 8:636-43.
4) Andersson By U, Tani E, Andersson U. Tumor necrosis factor, interleukin 11, and leukemia inhibitory factor produced by Langerhans cells in Langerhans cell histiocytosis. J. Pediatr. Hematol. Oncol. 2004; 26:706-11.

For Research Use Only