IL-11 Antibody [27B1047.1] Cat. No.: XP-5707-M

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Human
IMMUNOGEN: The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human IL-11 as the immunizing antigen.
TESTED APPLICATIONS: ELISA, Neut, WB
APPLICATIONS: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of human IL-11 (5.0 ng/mL), a concentration of 2.4 - 3.6 μg/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 8-9 μg/mL of this antibody will detect at least 0.2 ng/well of recombinant human IL-11 when used with our biotinylated antigen affinity purified anti-human IL-11(XP-5164Bt) as the detection antibody at a concentration of at least 2 μg/mL.

Western blot:

To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.

psi-iconProperties
PURIFICATION:This IgG1k antibody was purified from ascites fluid followed by Protein A affinity chromatography.
CLONALITY:Monoclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Lyophilized
STORAGE CONDITIONS:IL-11 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.

psi-iconAdditional Info
OFFICIAL SYMBOL:IL11
ALTERNATE NAMES:Interleukin 11Interleukin-11, Adipogenesis inhibitory factor, IL-11, AGIF
ACCESSION NO.:P20809
PROTEIN GI NO.:124294
GENE ID:3589
USER NOTE:Centrifuge vial prior to opening.

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