IL-10 Antibody [15B9-1-2] Cat. No.: 38-301

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Mouse
IMMUNOGEN: E.coli derived Recombinant Murine IL-10
TESTED APPLICATIONS: ELISA, Neut, WB
APPLICATIONS: ELISA:
Sandwich:
In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with ProSci’s Polyclonal biotinylated antigen affinity purified anti-murine IL-10 as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.

Western Blot:

To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.

Neutralization:

To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/mL), a concentration of 3.0-5.0 μg/mL of this antibody is required.

psi-iconProperties
PURIFICATION:This IgG2A antibody was purified from cell culture by Protein G affinity chromatography.
CLONALITY:Monoclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Lyophilized
STORAGE CONDITIONS:The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:IL10
ALTERNATE NAMES:Interleukin 10Interleukin-10, Cytokine synthesis inhibitory factor, IL-10, CSIF, TGIF, GVHDS, IL10A
ACCESSION NO.:NP_000563.1
PROTEIN GI NO.:10835141
GENE ID:3586
USER NOTE:Centrifuge vial prior to opening.

ANTIBODIES FOR RESEARCH USE ONLY.

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