Datasheet

IKK epsilon Antibody
CATALOG NUMBER: 2329

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (75%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:IKK epsilon antibody can be used for detection of IKK epsilon by Western blot at 1 μg/mL. An 80 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 1307 - Human Pancreas Tissue Lysate
 3) Cat. No. 11-101 - Human Pancreas Tissue Slide
PREDICTED MOLECULAR WEIGHT:80 kDa
SPECIFICITY:It has no cross response to IKKa, IKKb, or IKKy.
IMMUNOGEN:IKK epsilon antibody was raised against a 16 amino acid peptide near the carboxy terminus of human IKK epsilon.

The immunogen is located within the last 50 amino acids of IKK epsilon.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IKK epsilon Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IKK epsilon Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:IKK epsilon antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IKK epsilon Antibody: IKKE, IKKI, IKK-E, IKK-i, IKKE, KIAA0151, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, Inducible I kappa-B kinase, I-kappa-B kinase epsilon
ACCESSION NO.:AF241789
PROTEIN GI NO.:7288877
OFFICIAL SYMBOL:IKBKE
GENE ID:9641

Background

BACKGROUND:IKK epsilon Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB is phosphorylated by IκB kinase (IKK) complex that contains IKKα, IKKβ, and IKKγ. A novel molecule in the IKK complex was recently identified and designated IKKε/IKK-i. IKK epsilon is required for the activation of NF-κB by PMA and T cell receptors but not by TNFα and IL-1. IKKε/IKK-i message is expressed in a variety of tissues and is inducible by TNFα, IL-1, and LPS.
REFERENCES: 1) Peters RT, Liao SM, Maniatis T. IKK epsilonpsilon is part of a novel PMA-inducible IκB kinase complex. Mol Cell 2000;5(3):513-22
2) Shimada T, Kawai T, Takeda K, Matsumoto M, Inoue J, Tatsumi Y, Kanamaru A, Akira S. IKK-i, a novel lipopolysaccharide-inducible kinase that is related to IκB kinases. Int Immunol 1999;11(8):1357-62 (WD0101)

For Research Use Only