IKK epsilon Antibody Cat. No.: 2329

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (75%)
IMMUNOGEN: Anti-IKK epsilon antibody (2329) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IKK epsilon.

The immunogen is located within the last 50 amino acids of IKK epsilon.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 1-2 μg/mL; IHC: 10 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: It has no cross response to IKKa, IKKb, or IKKy.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 1307 - Human Pancreas Tissue Lysate
 3) Cat. No. 11-101 - Human Pancreas Tissue Slide
 4) Cat. No. 1202 - A431 Cell Lysate
 5) Cat. No. 1224 - Daudi Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 80kD

Observed: 80 kD
psi-iconAdvanced Validation
VALIDATION:

Induced Expression Validation (Figure 1): Raw 264.7 cells were treated with LPS (0.3 µg /mL) for different time period (3 hrs and 6 hrs). IKK epsilon expression detected by anit-IKK epsilon antibodies (2329) was induced by LPS treatment and the expression levels were increased gradually at 3 hrs and 6 hrs.

ISOFORMS: Human IKK epsilon has 2 isoforms, including isoform 1 (716aa, 80kD) and isoform 2 (631aa, 70kD). Mouse IKK epsilon has one isoform (717aa, 81kD) and Rat IKK epsilon also has one isoform (717aa, 80kD). 2329 can detect human, mouse and possibly rat.

psi-iconProperties
PURIFICATION:IKK epsilon Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:IKK epsilon Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/ml
STORAGE CONDITIONS:IKK epsilon antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:IKBKE
ALTERNATE NAMES:IKK epsilon Antibody: IKKE, IKKI, IKK-E, IKK-i, IKKE, KIAA0151, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, Inducible I kappa-B kinase, I-kappa-B kinase epsilon
ACCESSION NO.:AF241789
PROTEIN GI NO.:7288877
GENE ID:9641
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:IKK epsilon Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB is phosphorylated by IκB kinase (IKK) complex that contains IKKα, IKKβ, and IKKγ. A novel molecule in the IKK complex was recently identified and designated IKKε/IKK-i. IKK epsilon is required for the activation of NF-κB by PMA and T cell receptors but not by TNFα and IL-1. IKKε/IKK-i message is expressed in a variety of tissues and is inducible by TNFα, IL-1, and LPS.
REFERENCES:1) Peters et al. Mol Cell 2000;5(3):513-22.
2) Shimada et al. Int Immunol 1999;11(8):1357-62 (WD0101).

ANTIBODIES FOR RESEARCH USE ONLY.

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