This recombinant protein can be used for E, WB. For research use only.
BIOLOGICAL ACTIVITY:
Measured by its binding ability in a functional ELISA. Immobilized Rhesus macaque IGFBP-1, His Tag at 2 ug/mL (100 ul/well),can bind Human IGF-I, Fc Tag with a linear range of 2-16 ng/mL (QC tested).
Properties
PURITY:
>98% as determined by SDS-PAGE.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
PREDICTED MOLECULAR WEIGHT:
27.2 kDa
PHYSICAL STATE:
Lyophilized
BUFFER:
PBS, pH7.4
STORAGE CONDITIONS:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Additional Info
ALTERNATE NAMES:
IGFBP1,PP12,IBP1
ACCESSION NO.:
XP_001085935.2
OFFICIAL SYMBOL:
IGFBP-1
GENE ID:
696994
Background and References
BACKGROUND:
Insulin-like growth factor-binding protein 1 (IGFBP1) is also known as placental protein 12 (PP12), which contains oneIGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP1 can bindd both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of IGFBP1 can prolongd the half-life of the IGFs and alterd their interaction with cell surface receptors. Furthermore, IGFBP1 can promote cell migration. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
REFERENCES:
1) Lee Y.-L., et al., 1988, Mol. Endocrinol. 2:404-411.
2) Jones J.I., et al., 1993, J. Biol. Chem. 268:1125-1131.
3) Sala A., et al., 2005, J. Biol. Chem. 280:29812-29819.
Specifications
