Datasheet

IFN-beta Antibody
CATALOG NUMBER: 4243

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:IFN-b antibody can be used for detection of IFN-b by Western blot at 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1288 - A20 Cell Lysate
 2) Cat. No. 1304 - Human Liver Tissue Lysate
 3) Cat. No. 10-201 - Human Liver Tissue Slide
IMMUNOGEN:IFN-beta antibody was raised against a 17 amino acid synthetic peptide from near the center of human IFN-b.

The immunogen is located within amino acids 110 - 160 of IFN-beta.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IFN-beta Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IFN-beta Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IFN-beta antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IFN-beta Antibody: IFB, IFF, IFNB, IFB, Interferon beta, Fibroblast interferon, IFN-beta
ACCESSION NO.:AAC41702
PROTEIN GI NO.:184623
OFFICIAL SYMBOL:IFNB1
GENE ID:3456

Background

BACKGROUND:IFN-beta Antibody: Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by "Natural IFN-producing cells" (IPCs) as part of the host immune response and can also inhibit the development of tumors. IFN-beta binding by its receptor results in the activation of the tyrosine kinases Jak1 and Tyk2 and phosphorylation of members of the STAT family of transcription factors, leading to the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.
REFERENCES: 1) Gresser I. Wherefore interferon? J. Leuk. Biol.1997; 61:567-74.
2) Colonna M. TLR pathways and IFN-regulatory factors: to each its own. Eur. J. Immunol.2007; 37:306-9.
3) Kock M, Mostert J, De Keyser J, et al. Interferon-beta treatment and the natural history of relapsing-remitting multiple sclerosis. Ann. Neurol.2007; epub.

For Research Use Only