Datasheet

IFIT1 Antibody
CATALOG NUMBER: 7757

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:IFIT1 antibody can be used for detection of IFIT1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1468 - Rat Small Intestine Tissue Lysate
 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 53 kDa

Observed: 53 kDa
SPECIFICITY:IFIT1 antibody is human, mouse and rat reactive. At least three isoforms of IFIT1 are known to exist. This antibody is predicted to not cross-react with other members of the IFIT protein family.
IMMUNOGEN:IFIT1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human IFIT1.

The immunogen is located within amino acids 290 - 340 of IFIT1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IFIT1 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IFIT1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IFIT1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IFIT1 Antibody: C56, P56, G10P1, IFI56, ISG56, IFI-56, IFIT-1, IFNAI1, RNM561, IFI-56K, Interferon-induced protein with tetratricopeptide repeats 1, Interferon-induced 56 kDa protein
ACCESSION NO.:NP_001539
PROTEIN GI NO.:116534937
OFFICIAL SYMBOL:IFIT1
GENE ID:3434

Background

BACKGROUND:The interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) protein is a member of a family of tetratricopeptide repeat-containing proteins whose transcription is upregulated by interferons, virus infection, and molecular patterns such as dsRNA or lipopolysaccharides (1,2). These proteins have been suggested to induce anti-viral cellular activities in response to infection (2). Together with the interferon-induced transmembrane protein 1 (IFITM1), IFIT1 inhibits the replication of the Hepatitis C virus, suggesting that it may be useful in the development of therapeutic treatments (3).
REFERENCES: 1) Wathelet M, Moutschen S, Defilippi P, et al. Molecular cloning, full-length sequence and preliminary characterization of a 56-kDa protein induced by human interferons. Eur. J. Biochem. 1986; 155:11-7.
2) Fensterl V and Sen GC. The ISG56/IFIT1 gene family. J. Interferon Cytokine Res. 2011; 31:71-8.
3) Raychoudhuri A, Shrivastava S, Steele R, et al. ISG56 and IFITM1 proteins inhibit hepatitis C virus replication. J. Virol. 2011; 85:12881-9.

For Research Use Only