IDH2 Antibody Cat. No.: 5821

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (88%)
IMMUNOGEN: IDH2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human IDH2.

The immunogen is located within the first 50 amino acids of IDH2.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: IDH2 antibody can be used for detection of IDH2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1301 - Human Heart Tissue Lysate

psi-iconProperties
PURIFICATION:IDH2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:IDH2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IDH2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:IDH2
ALTERNATE NAMES:IDH2 Antibody: IDH, IDP, IDHM, IDPM, ICD-M, D2HGA2, mNADP-IDHICD-M, IDH
ACCESSION NO.:NP_002159
PROTEIN GI NO.:28178832
GENE ID:3418
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:IDH2 Antibody: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Two NADP(+)-dependent isocitrate dehydrogenases have been found as homodimer: IDH1 is predominantly cytosolic and peroxisomal and IDH2 is mitochondrial. Both IDH1 and IDH2 play significant roles in the tricarboxylic acid cycle (TCA), and defects in IDH1 as well as IDH2 have been implicated in the development of glioma as well as other malignancies.
REFERENCES:1) Geisbrecht BV and Gould SJ. The human PICD gene encodes a cytoplasmic and peroxisomal NADP(+)-dependent isocitrate dehydrogenase. J. Biol. Chem.1999; 274:30527-33.
2) Przybyla-Zawislak B, Gadde DM, Ducharme K, et al. Genetic and biochemical interactions involving tricarboxylic acid cycle (TCA) function using a collection of mutants defective in all TCA cycle genes. Genetics1999; 152:153-66.
3) Dang L, White DW, and Gross S. Cancer-associated IDH1 mutations produce 2-hydroxyglutarate. Nature2009; 462:739-44.
4) Tan H, Parsons DW, Jin G, et al. IDH1 and IDH2 mutations in gliomas. N. Engl. J. Med.2009; 360:765-73.

ANTIBODIES FOR RESEARCH USE ONLY.

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