Datasheet

IDH1 Antibody
CATALOG NUMBER: 5819

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Sheep: (100%), Bovine: (100%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:IDH1 antibody can be used for detection of IDH1 by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1211 - HepG2 Cell Lysate
IMMUNOGEN:IDH1 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of human IDH1.

The immunogen is located within the last 50 amino acids of IDH1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IDH1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IDH1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IDH1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IDH1 Antibody: IDH, IDP, IDCD, IDPC, PICD, HEL-216, HEL-S-26, Cytosolic NADP-isocitrate dehydrogenase, IDH
ACCESSION NO.:NP_005887
PROTEIN GI NO.:28178825
OFFICIAL SYMBOL:IDH1
GENE ID:3417

Background

BACKGROUND:IDH1 Antibody: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Two NADP(+)-dependent isocitrate dehydrogenases have been found as homodimer: IDH1 is predominantly cytosolic and peroxisomal and IDH2 is mitochondrial. The presence of IDH1 in peroxisomes suggests it may play a role in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic IDH1 serves a significant role in cytoplasmic NADPH production. Defects in IDH1 are involved in the development of glioma.
REFERENCES: 1) Geisbrecht BV and Gould SJ. The human PICD gene encodes a cytoplasmic and peroxisomal NADP(+)-dependent isocitrate dehydrogenase. J. Biol. Chem.1999; 274:30527-33.
2) Xu X, Zhao J, Xu Z, et al. Structures of human cytosolic NADP-dependent isocitrate dehydrogenase reveal a novel self-regulatory mechanism of activity. J. Biol. Chem.2004; 279:33946-57.
3) Dang L, White DW, and Gross S. Cancer-associated IDH1 mutations produce 2-hydroxyglutarate. Nature2009; 462:739-44.
4) Smeitink J. Metabolism, gliomas, and IDH1. N. Engl. J. Med.2010; 362:1144-5.

For Research Use Only