Datasheet

IA-1 Antibody
CATALOG NUMBER: 5415

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:IA-1 antibody can be used for detection of IA-1 by Western blot at 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1471 - Rat Thymus Tissue Lysate
IMMUNOGEN:IA-1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human IA-1.

The immunogen is located within amino acids 90 - 140 of IA-1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IA-1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IA-1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IA-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IA-1 Antibody: IA1, IA-1, IA1, Insulinoma-associated protein 1, Zinc finger protein IA-1
ACCESSION NO.:NP_002187
PROTEIN GI NO.:4504713
OFFICIAL SYMBOL:INSM1
GENE ID:3642

Background

BACKGROUND:IA-1 Antibody: IA-1, also known as INSM1, is an essential five zinc-finger transcription factor that while initially identified from an insulinoma cDNA library, is expressed in the developing nervous system. Specifically, IA-1 is expressed as early as E9.5 in mice in the fore-, mid- and hindbrain, spinal cord, retina, and olfactory bulb. It is thought that IA-1 functions as a transcriptional repressor, and is a part of several signaling pathways including those of Notch and sonic hedgehog in addition to that of Ngn3 during pancreatic endocrine cell differentiation. IA-1 is also expressed in multiple tumors, including the majority of neuroendocrine tumors tested so far as well as nearly 100% of all small cell lung carcinomas, indicating that IA-1 may be an important target in cancer therapy.
REFERENCES: 1) Lan MS, Li Q, Lu J, et al. Genomic organization, 5’-upstream sequence, and chromosomal localization of an insulinoma-associated intronless gene, IA-1. J. Biol. Chem.1994; 269:14170-4.
2) Jacob J, Storm R, Castro DS, et al. Insm (IA-1) is an essential component of the regulatory network that specifies monoaminergic neuronal phenotypes in the vertebrate hindbrain. Dev.2009; 136:2477-85.
3) Breslin MB, Zhu M, and Lan MS. NeuroD1/E47 regulates the E-box element of a novel zinc-finger transcription factor, IA-1, in developing nervous system. J. Biol. Chem.2003; 278:38991-7.
4) Nelson BR, Hartman BH, Georgi SA, et al. Transient inactivation of Notch signaling synchronizes differentiation of neural progenitor cells. Dev. Biol.2007; 304:479-98.

For Research Use Only