Datasheet

HVCN1 Antibody
CATALOG NUMBER: 5875

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (71%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:HVCN1 antibody can be used for detection of HVCN1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1406 - Mouse Spleen Tissue Lysate
 2) Cat. No. 1306 - Human Spleen Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 30 kDa

Observed: 30 kDa
IMMUNOGEN:HVCN1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human HVCN1.

The immunogen is located within the last 50 amino acids of HVCN1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:HVCN1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:HVCN1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:HVCN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:HVCN1 Antibody: HV1, VSOP, UNQ578/PRO1140, Voltage-gated hydrogen channel 1, Hydrogen voltage-gated channel 1, HV1
ACCESSION NO.:NP_115745
PROTEIN GI NO.:91992155
OFFICIAL SYMBOL:HVCN1
GENE ID:84329

Background

BACKGROUND:HVCN1 Antibody: HVCN1 (hydrogen voltage-gated channel 1), a voltage gated proton channel protein that is highly expressed in immune tissues, mediates the proton conductances required by phagocytic leukocytes for the oxidative burst that underlies microbial killing. HVCN1 moderates the voltage-dependent proton permeability of excitable membranes by allowing the flow of protons in accordance to their electrochemical gradient. HVCN1 is sensitive to zinc ions, and can be inhibited by them. HVCN1 may represent a new therapeutic target for B cell malignancies on continued signaling via the B cell antigen receptor.
REFERENCES: 1) Ramsey IS, Moran MM, Chong JA, et al. A voltage gated proton-selective channel lacking the pore domain. Nature2006; 440:1213-6.
2) Sasaki M, Takagi M, and Okamura Y. A voltage sensor-domain protein is a voltage-gated proton channel. Science2006; 312: 589-92.
3) Morgan D, Capasso M, and Musset B. Voltage-gated proton channels maintain pH in human neutrophils during phagocytosis. Proc. Natl. Acad. Sci. USA2009; 106:18022-7.
4) Capasso M, Bhamrah MK, Henley T, et al. HVCN1 modulates BCR signal strength via regulation of BCR-dependent generation of reactive oxygen species. Nat. Immunol.2010;11:265-72.

For Research Use Only