Human Coronavirus Nucleocapsid (229E) Recombinant Protein Cat. No.: 20-201

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psi-iconSpecifications
SPECIES:HCoV-OC43
SOURCE SPECIES:bacteria
SEQUENCE:Native Sequence:
MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGPLGSMATVKWADASEPQRGRQGRIPYSLYSPLLVDSEQPWKVIPRNLVPINKKDKNKLIGYWNVQKRFRTRKGKRVDLSPKLHFYYLGTGPHKDAKFRERVEGVVWVAVDGAKTEPTGYGVRRKNSEPEIPHFNQKLPNGVTVVEEPDSRAPSRSQSRSQSRGRGESKPQSRNPSSDRNHNSQDDIMKAVAAALKSLGFDKPQEKDKKSAKTGTPKPSRNQSPASSQTSAKSLARSQSSETKEQKHEMQKPRWKRQPNDDVTSNVTQCFGPRDLDHNFGSAGVVANGVKAKGYPQFAELVPSTAAMLFDSHIVSKESGNTVVLTFTTRVTVPKDHPHLGKFLEELNAFTREMQQHPLLNPSALEFNPSQTSPATAEPVRDEVSIETDIIDEVN

Amino acids M1 – N389 (end).
Residue M404 of the fusion protein is equivalent to M1 of the native enzyme.
The MBP tag is located at residues 1 – 367.

Protease Cleavage:
PreScission (LEVLFQGP) residues 393 - 400
FUSION TAG:N-Term MBP Uncleaved
psi-iconProperties
PURITY:Amylose Resin (0.85)
PREDICTED MOLECULAR WEIGHT:Mono-Isotopic Mass: 87, 651.64 daltons
Average Mass: 87, 705.68 daltons
PHYSICAL STATE:Liquid
BUFFER:50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35
CONCENTRATION:batch dependent
STORAGE CONDITIONS:Store at -70 °C. Avoid repeated freeze-thaw cycles.
psi-iconAdditional Info
ALTERNATE NAMES:Human CoV 229E N Protein, Human CoV 229E Nucleocapsid Protein
ACCESSION NO.:NP_073556.1
OFFICIAL SYMBOL:N
GENE ID:918763

FOR RESEARCH USE ONLY.

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