hRIP3 Antibody Cat. No.: 8963

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: Anti-hRIP3 antibody (8963) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human hRIP3.

The immunogen is located within the last 50 amino acids of hRIP3.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 0.5-1 μ g/mL; IF: 20 μ g/mL; IHC: 2-5 μ g/mL.

Antibody validated: Western Blot, Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested.
SPECIFICITY: Several isoforms of hRIP3 are known to exist.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 1224 - Daudi Cell Lysate
 3) Cat. No. 1208 - THP-1 Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 57kD

Observed: 57 kD

psi-iconProperties
PURIFICATION:hRIP3 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:hRIP3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/ml
STORAGE CONDITIONS:hRIP3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:Ripk3
ALTERNATE NAMES:hRIP3 Antibody: Rip3, AW107945, 2610528K09Rik, RIP-like protein kinase 3, RIP-3
ACCESSION NO.:Q9Y572
PROTEIN GI NO.:205371831
GENE ID:11035
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:hRIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.
REFERENCES:1) Yu et al. Curr Biol. 1999;9(10):539-42.
2) Sun et al. J Biol Chem. 1999;274(24):16871-5.
3) Pazdernik et al. Mol Cell Bio. 1999; 19(10):6500-8 (WD0102)

ANTIBODIES FOR RESEARCH USE ONLY.

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