HOST SPECIES: | Rabbit |
SPECIES REACTIVITY: | Human |
IMMUNOGEN: | Anti-hRIP3 antibody (8963) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human hRIP3. The immunogen is located within the last 50 amino acids of hRIP3. |
TESTED APPLICATIONS: | ELISA, IF, IHC-P, WB |
APPLICATIONS: | WB: 0.5-1 μg/mL; IF: 20 μg/mL; IHC: 2-5 μg/mL. Antibody validated: Western Blot, Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested. |
SPECIFICITY: | Several isoforms of hRIP3 are known to exist. |
POSITIVE CONTROL: | 1) Cat. No. 1205 - Jurkat Cell Lysate |
2) Cat. No. 1224 - Daudi Cell Lysate | |
3) Cat. No. 1208 - THP-1 Cell Lysate | |
PREDICTED MOLECULAR WEIGHT: | Predicted: 57kD Observed: 57 kD |
PURIFICATION: | hRIP3 Antibody is affinity chromatography purified via peptide column. |
CLONALITY: | Polyclonal |
ISOTYPE: | IgG |
CONJUGATE: | Unconjugated |
PHYSICAL STATE: | Liquid |
BUFFER: | hRIP3 Antibody is supplied in PBS containing 0.02% sodium azide. |
CONCENTRATION: | 1 mg/ml |
STORAGE CONDITIONS: | hRIP3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
OFFICIAL SYMBOL: | Ripk3 |
ALTERNATE NAMES: | hRIP3 Antibody: Rip3, AW107945, 2610528K09Rik, RIP-like protein kinase 3, RIP-3 |
ACCESSION NO.: | Q9Y572 |
PROTEIN GI NO.: | 205371831 |
GENE ID: | 11035 |
USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
BACKGROUND: | hRIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues. |
REFERENCES: | 1) Yu et al. Curr Biol. 1999;9(10):539-42. | 2) Sun et al. J Biol Chem. 1999;274(24):16871-5. | 3) Pazdernik et al. Mol Cell Bio. 1999; 19(10):6500-8 (WD0102) |
ANTIBODIES FOR RESEARCH USE ONLY.
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