HOPX Antibody Cat. No.: 8143

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (88%), Mouse: (88%), Bovine: (88%), Pig: (81%), Chicken: (81%)
IMMUNOGEN: HOPX antibody was raised against a 16 amino acid peptide near the amino terminus of human HOPX.

The immunogen is located within the first 50 amino acids of HOPX.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: HOPX antibody can be used for detection of HOPX by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: HOPX antibody is human specific. At least three isoforms of HOPX are known to exist; this antibody will detect all three isoforms.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
 2) Cat. No. 10-501 - Human Heart Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 12 kDa

Observed: 18 kDa

psi-iconProperties
PURIFICATION:HOPX antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:HOPX antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:HOPX antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:HOPX
ALTERNATE NAMES:HOP homeobox, homeodomain-only protein, CAMEO, HOD, HOP, LAGY, NECC1, OB1, SMAP31, TOTO
ACCESSION NO.:NP_001138932
PROTEIN GI NO.:224451025
GENE ID:84525
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:HOPX is a small homeodomain protein that lacks normally conserved residues required for DNA binding (1). It is thought to act downstream of NKX2-5 and modulates serum response factor (SRF)-dependent cardiac-specific gene expression and cardiac development (1). HOPX also acts a tumor suppressor gene in multiple tumors and cancer cell lines (2,3). HOPX has been reported to regulate the proliferation/differentiation homeostasis in different cell types, including keratinocytes (4).
REFERENCES:1) Chen F, Kook H, Milewski R, et al. Hop is an unusual homeobox gene that modulates cardiac development. Cell 2002; 110:713-23.
2) Asanoma K, Matsuda T, Kondo H, et al. NECC1, a candidate choriocarcinoma suppressor gene that encodes a homeodomain consensus motif. Genomics 2003; 81:15-25.
3) Katoh H, Yamashita K, Waraya M, et al. Epigenetic silencing of HOPX promotes cancer progression in colorectal cancer. Neoplasia 2012; 14:559-71.
4) Obarzanek-Fojt M, Favre B, Kypriotou M, et al. Homeodomain-only protein HOP is a novel modulator of late differentiation in keratinocytes. Eur. J. Cell Biol. 2011; 90:279-90.

ANTIBODIES FOR RESEARCH USE ONLY.

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