HOOK3 Antibody Cat. No.: 8425

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: HOOK3 antibody was raised against a 19 amino acid peptide from near the amino terminus of human HOOK3.

The immunogen is located within amino acids 210 - 260 of HOOK3.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: HOOK3 antibody can be used for the detection of HOOK3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry at 10 μg/ml.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: HOOK3 antibody is human, mouse and rat reactive. HOOK3 antibody is predicted to not cross-react with HOOK1 and HOOK2.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 79 kDa

Observed: 77 kDa

psi-iconProperties
PURIFICATION:HOOK3 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:HOOK3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:HOOK3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:HOOK3
ALTERNATE NAMES:hook microtubule-tethering protein 3, HK3, h-hook3, hHK3, FLJ31058, Protein Hook homolog 3
ACCESSION NO.:NP_115786
PROTEIN GI NO.:14165274
GENE ID:84376
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The HOOK proteins (HOOK1, HOOK2 and HOOK3) comprise a family of cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules mediating the spatial organization of diverse membrane-trafficking systems; their more divergent C-terminal domains mediate binding to organelles (1,2). HOOK3 exists as a homodimer and participates in defining the architecture and localization of the Golgi complex through its central coiled-coil domain (3). HOOK3 may regulate clearance of endocytosed receptors such as MSR1 (3,4).
REFERENCES:1) Walenta JH, Didier AJ, Liu X, et al. The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins. J. Cell Biol. 2001; 152:923-34.
2) Sano H, Ishino M, Krämer H, et al. The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A. J. Biol. Chem. 2007; 282:7973-81.
3) Herrmann L, Wiegmann C, Arsalan-Werner A, et al. Hook proteins: association with Alzheimer pathology and regulatory role of hook3 in amyloid beta generation. PLoS One 2015; 10:e0119423.
4) Ciampi R, Giordano TJ, Wikenheiser-Brokamp K, et al. HOOK3-RET: a novel type of RET/PTC rearrangement in papillary thyroid carcinoma. Endocr. Relat. Cancer 2007; 14:445-52.

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