HMGB1 Antibody, KO Validated Cat. No.: 18-706

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (NP_002119.1).
TESTED APPLICATIONS: IF, IHC, WB
APPLICATIONS: WB: ,1:500 - 1:2000

IHC: ,1:50 - 1:200

IF: ,1:50 - 1:200
POSITIVE CONTROL:1) 293T
 2) MCF7
 3) Jurkat
 4) Mouse spleen
 5) Mouse lung
 6) Mouse kidney
PREDICTED MOLECULAR WEIGHT: Observed: 29kDa
psi-iconAdvanced Validation
VALIDATION: Antibody is Knockout validated.

psi-iconProperties
PURIFICATION:Affinity purification
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
STORAGE CONDITIONS:Store at -20˚C. Avoid freeze / thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:HMGB1
ALTERNATE NAMES:HMGB1 Antibody: HMG1, HMG3, SBP-1, HMG1, High mobility group protein B1, High mobility group protein 1, HMG-1
GENE ID:3146
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

ANTIBODIES FOR RESEARCH USE ONLY.

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