Datasheet

HMGB1 Antibody
CATALOG NUMBER: 7715

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (100%), Pig: (100%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:HMGB1 antibody can be used for detection of HMGB1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
 2) Cat. No. 10-101 - Human Lung Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 24 kDa

Observed: 24 kDa
SPECIFICITY:HMGB1 antibody is human, mouse and rat reactive.
IMMUNOGEN:HMGB1 antibody was raised against a 19 amino acid peptide near the center of human HMGB1.

The immunogen is located within amino acids 70 - 120 of HMGB1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:HMGB1 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:HMGB1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:HMGB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:HMGB1 Antibody: HMG1, HMG3, SBP-1, HMG1, High mobility group protein B1, High mobility group protein 1, HMG-1
ACCESSION NO.:NP_002119
PROTEIN GI NO.:4504425
OFFICIAL SYMBOL:HMGB1
GENE ID:3146

Background

BACKGROUND:The high-mobility group box 1 (HMGB1) protein was originally identified as a highly conserved nuclear DNA-binding protein that participates in DNA replication, repair and transcriptional regulation of gene expression (1,2). However, HMGB1 has more recently emerged as an extracellularly released mediator of inflammation and repair responses in lipopolysaccharide (LPS)-induced endotoxemia and sepsis (3). HMGB1 has also been suggested to induce secretion of pro-inflammatory cytokines and chemokines by inducing NF-kB signaling downstream of Toll-like receptor (TLR) 2 and TLR4 activation (4).
REFERENCES: 1) Smerdon MJ and Isenberg I. Interactions between the subfractions of calf thymus H1 and nonhistone chromosomal proteins HMG1 and HMG2. Biochemistry 1976; 15:4242-7.
2) Stros M. HMGB proteins: interactions with DNA and chromatin. Biochim. Biophys. Acta. 2010; 1799:101-13.
3) Wang H, Bloom O, Zhang M, , et al. HMG-1 as a late mediator of endotoxin lethality in mice. Science 1999; 285:248-51.
4) van Zoelen MA, Yang H, Florquin S, et al. Role of toll-like receptors 2 and 4, and the receptor for advanced glycation end products in high-mobility group box 1-induced inflammation in vivo. Shock 2009; 31:280-4.

For Research Use Only